Exploring the Wound Healing, Anti-inflammatory, Anti-pathogenic and Proteomic Effects of Lactic Acid Bacteria on Keratinocytes

Overview

Journal Sci Rep

Specialty Science

Date 2020 Jul 16

PMID 32665600

Citations 48

Authors

Jessica Brandi

Samuele Cheri

Marcello Manfredi

Claudia Di Carlo

Virginia Vita Vanella

Federica Federici

Eleonora Bombiero

Alda Bazaj

Eleonora Rizzi

Laura Manna

Giuseppe Cornaglia

Umberto Marini

Maria Teresa Valenti

Emilio Marengo

Daniela Cecconi

Affiliations

Soon will be listed here.

Abstract

The topical application of lactic acid bacteria (LAB) is recognized as a useful approach to improve skin health. This work aims to characterize by a multidisciplinary approach, the wound healing, anti-inflammatory, anti-pathogens and proteomic effects of six LAB lysates, belonging to the genus Lactobacillus. Our results demonstrated that the lysates of tested LAB stimulated the proliferation of keratinocytes, and that L. plantarum SGL 07 and L. salivarius SGL 19 accelerated the re-epithelization by inducing keratinocyte migration. The bacterial lysates also reduced the secretion of specific pro-inflammatory mediators from keratinocytes. Furthermore, viable L. salivarius SGL 19 and L. fermentum SGL 10 had anti-pathogenic effects against S. aureus and S. pyogenes, while L. brevis SGL 12 and L. paracasei SGL 04 inhibited S. aureus and S. pyogenes, respectively. The tested lactobacilli lysates also induced specific proteome modulation of the exposed keratinocytes, involving dysregulation of proteins (such as interleukin enhancer-binding factor 2 and ATP-dependent RNA helicase) and pathways (such as cytokine, NF-kB, Hedgehog, and RUNX signaling) associated with their specific wound healing and anti-inflammatory effects. This study indicates the different potential of selected lactobacilli, suggesting that they may be successfully used in the future together with conventional therapies to bring relief from skin disorders.

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