Pupil Engineering for Extended Depth-of-field Imaging in a Fluorescence Miniscope

Overview

Journal Neurophotonics

Date 2023 May 22

PMID 37215637

Authors

Joseph Greene

Yujia Xue

Jeffrey Alido

Alex Matlock

Guorong Hu

Kivilcim Kilic

Ian Davison

Lei Tian

Affiliations

Soon will be listed here.

Abstract

Significance: Fluorescence head-mounted microscopes, i.e., miniscopes, have emerged as powerful tools to analyze neural populations but exhibit a limited depth-of-field (DoF) due to the use of high numerical aperture (NA) gradient refractive index (GRIN) objective lenses.

Aim: We present extended depth-of-field (EDoF) miniscope, which integrates an optimized thin and lightweight binary diffractive optical element (DOE) onto the GRIN lens of a miniscope to extend the DoF by between twin foci in fixed scattering samples.

Approach: We use a genetic algorithm that considers the GRIN lens' aberration and intensity loss from scattering in a Fourier optics-forward model to optimize a DOE and manufacture the DOE through single-step photolithography. We integrate the DOE into EDoF-Miniscope with a lateral accuracy of to produce high-contrast signals without compromising the speed, spatial resolution, size, or weight.

Results: We characterize the performance of EDoF-Miniscope across 5- and fluorescent beads embedded in scattering phantoms and demonstrate that EDoF-Miniscope facilitates deeper interrogations of neuronal populations in a -thick mouse brain sample and vessels in a whole mouse brain sample.

Conclusions: Built from off-the-shelf components and augmented by a customizable DOE, we expect that this low-cost EDoF-Miniscope may find utility in a wide range of neural recording applications.

Citing Articles

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