A One-photon Endoscope for Simultaneous Patterned Optogenetic Stimulation and Calcium Imaging in Freely Behaving Mice

Overview

Journal Nat Biomed Eng

Publisher Springer Nature

Specialty Biomedical Engineering

Date 2022 Aug 15

PMID 35970930

Authors

Jinyong Zhang

Ryan N Hughes

Namsoo Kim

Isabella P Fallon

Konstantin Bakhurin

Jiwon Kim

Francesco Paolo Ulloa Severino

Henry H Yin

Affiliations

Soon will be listed here.

Abstract

Optogenetics and calcium imaging can be combined to simultaneously stimulate and record neural activity in vivo. However, this usually requires two-photon microscopes, which are not portable nor affordable. Here we report the design and implementation of a miniaturized one-photon endoscope for performing simultaneous optogenetic stimulation and calcium imaging. By integrating digital micromirrors, the endoscope makes it possible to activate any neuron of choice within the field of view, and to apply arbitrary spatiotemporal patterns of photostimulation while imaging calcium activity. We used the endoscope to image striatal neurons from either the direct pathway or the indirect pathway in freely moving mice while activating any chosen neuron in the field of view. The endoscope also allows for the selection of neurons based on their relationship with specific animal behaviour, and to recreate the behaviour by mimicking the natural neural activity with photostimulation. The miniaturized endoscope may facilitate the study of how neural activity gives rise to behaviour in freely moving animals.

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