Changes in the Salivary Metabolome in Patients with Chronic Erosive Gastritis

Overview

Journal BMC Gastroenterol

Publisher Biomed Central

Specialty Gastroenterology

Date 2023 May 19

PMID 37208605

Authors

Shaowei Liu

Shixiong Zhang

Haoyu Chen

Pingping Zhou

Tianxiao Yang

Jingjing Lv

Huixia Li

Yangang Wang

Affiliations

Soon will be listed here.

Abstract

Introduction: Chronic erosive gastritis (CEG) is closely related to gastric cancer, which requires early diagnosis and intervention. The invasiveness and discomfort of electronic gastroscope have limited its application in the large-scale screening of CEG. Therefore, a simple and noninvasive screening method is needed in the clinic.

Objectives: The aim of this study is to screen potential biomarkers that can identify diseases from the saliva samples of CEG patients using metabolomics.

Methods: Saliva samples from 64 CEG patients and 30 healthy volunteers were collected, and metabolomic analysis was performed using UHPLC-Q-TOF/MS in the positive and negative ion modes. Statistical analysis was performed using both univariate (Student's t-test) and multivariate (orthogonal partial least squares discriminant analysis) tests. Receiver operating characteristic (ROC) analysis was conducted to determine significant predictors in the saliva of CEG patients.

Results: By comparing the saliva samples from CEG patients and healthy volunteers, 45 differentially expressed metabolites were identified, of which 37 were up-regulated and 8 were down-regulated. These differential metabolites were related to amino acid, lipid, phenylalanine metabolism, protein digestion and absorption, and mTOR signaling pathway. In the ROC analysis, the AUC values of 7 metabolites were greater than 0.8, among which the AUC values of 1,2-dioleoyl-sn-glycoro-3-phosphodylcholine and 1-stearoyl-2-oleoyl-sn-glycoro-3-phospholine (SOPC) were greater than 0.9.

Conclusions: In summary, a total of 45 metabolites were identified in the saliva of CEG patients. Among them, 1,2-dioleoyl-sn-glycoro-3-phosphorylcholine and 1-stearoyl-2-oleoyl-sn-glycoro-3-phosphorine (SOPC) might have potential clinical application value.

Citing Articles

Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens.

de Souza H, Pereira T, de Sa H, Alves M, Garrett R, Canuto G Metabolites. 2024; 14(1).

PMID: 38248839 PMC: 10819689. DOI: 10.3390/metabo14010036.

Study on the Characteristics of Traditional Chinese Medicine Syndromes in Patients with Erosive Gastritis Based on Metabolomics.

Shixiong Z, Shaowei L, Zeqi Y, Miaochan X, Pingping Z, Haiyan B Int J Anal Chem. 2024; 2024:6684677.

PMID: 38204992 PMC: 10776191. DOI: 10.1155/2024/6684677.

References

Hollander D, Tarnawski A . Is there a role for dietary essential fatty acids in gastroduodenal mucosal protection?. J Clin Gastroenterol. 1991; 13 Suppl 1:S72-4. DOI: 10.1097/00004836-199112001-00012. View

Kanehisa M, Furumichi M, Sato Y, Kawashima M, Ishiguro-Watanabe M . KEGG for taxonomy-based analysis of pathways and genomes. Nucleic Acids Res. 2022; 51(D1):D587-D592. PMC: 9825424. DOI: 10.1093/nar/gkac963. View

Farthing M, Fairclough P, Hegarty J, Swarbrick E, Dawson A . Treatment of chronic erosive gastritis with prednisolone. Gut. 1981; 22(9):759-62. PMC: 1419870. DOI: 10.1136/gut.22.9.759. View

Benton H, Ivanisevic J, Mahieu N, Kurczy M, Johnson C, Franco L . Autonomous metabolomics for rapid metabolite identification in global profiling. Anal Chem. 2014; 87(2):884-91. PMC: 4303330. DOI: 10.1021/ac5025649. View

Chang W, Bai J, Tian S, Ma M, Li W, Yin Y . Autophagy protects gastric mucosal epithelial cells from ethanol-induced oxidative damage via mTOR signaling pathway. Exp Biol Med (Maywood). 2017; 242(10):1025-1033. PMC: 5444638. DOI: 10.1177/1535370216686221. View

Arab H, Ashour A, Gad A, Mahmoud A, Kabel A . Activation of AMPK/mTOR-driven autophagy and inhibition of NLRP3 inflammasome by saxagliptin ameliorate ethanol-induced gastric mucosal damage. Life Sci. 2021; 280:119743. DOI: 10.1016/j.lfs.2021.119743. View

Zheng H, Zhang X, Li L, Wang Y, Wei Y, Chen Y . Multicentre prospective cohort study evaluating gastroscopy without sedation in China. Br J Anaesth. 2018; 121(2):508-511. DOI: 10.1016/j.bja.2018.04.027. View

Huang S, Guo Y, Li Z, Shui G, Tian H, Li B . Identification and Validation of Plasma Metabolomic Signatures in Precancerous Gastric Lesions That Progress to Cancer. JAMA Netw Open. 2021; 4(6):e2114186. PMC: 8220475. DOI: 10.1001/jamanetworkopen.2021.14186. View

Mocellin M, Fernandes R, Chagas T, Trindade E . A meta-analysis of n-3 polyunsaturated fatty acids effects on circulating acute-phase protein and cytokines in gastric cancer. Clin Nutr. 2017; 37(3):840-850. DOI: 10.1016/j.clnu.2017.05.008. View

10.

Wei F, Lin C, Joon A, Feng Z, Troche G, Lira M . Noninvasive saliva-based EGFR gene mutation detection in patients with lung cancer. Am J Respir Crit Care Med. 2014; 190(10):1117-26. PMC: 5447327. DOI: 10.1164/rccm.201406-1003OC. View

11.

Park J, Han Y, Jeong M, Kim E, Ko W, Cho J . Omega-3 polyunsaturated fatty acids as an angelus custos to rescue patients from NSAID-induced gastroduodenal damage. J Gastroenterol. 2015; 50(6):614-25. DOI: 10.1007/s00535-014-1034-z. View

12.

Mohamed R, Varesio E, Ivosev G, Burton L, Bonner R, Hopfgartner G . Comprehensive analytical strategy for biomarker identification based on liquid chromatography coupled to mass spectrometry and new candidate confirmation tools. Anal Chem. 2009; 81(18):7677-94. DOI: 10.1021/ac901087t. View

13.

Juhasz M, Nagy V, Szekely H, Kocsis D, Tulassay Z, Laszlo J . Influence of inhomogeneous static magnetic field-exposure on patients with erosive gastritis: a randomized, self- and placebo-controlled, double-blind, single centre, pilot study. J R Soc Interface. 2014; 11(98):20140601. PMC: 4233709. DOI: 10.1098/rsif.2014.0601. View

14.

Aitken J, Ortiz C, Morales-Bozo I, Rojas-Alcayaga G, Baeza M, Beltran C . α-2-macroglobulin in saliva is associated with glycemic control in patients with type 2 diabetes mellitus. Dis Markers. 2015; 2015:128653. PMC: 4363888. DOI: 10.1155/2015/128653. View

15.

Zhou P, Hao X, Liu Y, Yang Z, Xu M, Liu S . Determination of the protective effects of Hua-Zhuo-Jie-Du in chronic atrophic gastritis by regulating intestinal microbiota and metabolites: combination of liquid chromatograph mass spectrometer metabolic profiling and 16S rRNA gene sequencing. Chin Med. 2021; 16(1):37. PMC: 8088729. DOI: 10.1186/s13020-021-00445-y. View

16.

Ferraz J, Tigley A, Wallace J . Paradoxical effects of L-arginine on gastric mucosal integrity. Eur J Pharmacol. 1994; 260(1):107-11. DOI: 10.1016/0014-2999(94)90018-3. View

17.

Chen S, Mo J, Cao Z, Chen X, Xiao S . Effects of bile reflux on gastric mucosal lesions in patients with dyspepsia or chronic gastritis. World J Gastroenterol. 2005; 11(18):2834-7. PMC: 4305928. DOI: 10.3748/wjg.v11.i18.2834. View

18.

Zhang C, Cheng X, Li J, Zhang P, Yi P, Xu X . Saliva in the diagnosis of diseases. Int J Oral Sci. 2016; 8(3):133-7. PMC: 5113094. DOI: 10.1038/ijos.2016.38. View

19.

Kosaka T, Kokubo Y, Ono T, Sekine S, Kida M, Kikui M . Salivary inflammatory cytokines may be novel markers of carotid atherosclerosis in a Japanese general population: the Suita study. Atherosclerosis. 2014; 237(1):123-8. DOI: 10.1016/j.atherosclerosis.2014.08.046. View

20.

Hizir M, Balcioglu M, Rana M, Robertson N, Yigit M . Simultaneous detection of circulating oncomiRs from body fluids for prostate cancer staging using nanographene oxide. ACS Appl Mater Interfaces. 2014; 6(17):14772-8. DOI: 10.1021/am504190a. View