Immune Serum to Protein Molecular Weight Standards for Calibrating Western Blots

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1986 May 15

PMID 3717561

Citations 4

Authors

G M Carlone

B B Plikaytis

R J Arko

Affiliations

Soon will be listed here.

Abstract

A method for calibrating proteins by Western blot analysis is described. Rabbit immune serum, specific for seven protein molecular weight standards, and goat anti-rabbit horseradish peroxidase conjugate are used to visualize standards after electrotransfer onto nitrocellulose. This method can be used as a reagent and procedure control.

Citing Articles

Purification, partial characterization, and seroreactivity of a genuswide 60-kilodalton Legionella protein antigen.

Pau C, Plikaytis B, Carlone G, Warner I J Clin Microbiol. 1988; 26(1):67-71.

PMID: 3343316 PMC: 266188. DOI: 10.1128/jcm.26.1.67-71.1988.

Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes.

Plikaytis B, Carlone G, Pau C, WILKINSON H J Clin Microbiol. 1987; 25(11):2080-4.

PMID: 2447117 PMC: 269415. DOI: 10.1128/jcm.25.11.2080-2084.1987.

Monoclonal antibody recognizing a species-specific protein from Streptococcus pneumoniae.

Russell H, Tharpe J, Wells D, White E, Johnson J J Clin Microbiol. 1990; 28(10):2191-5.

PMID: 2229341 PMC: 268145. DOI: 10.1128/jcm.28.10.2191-2195.1990.

Immunologic characterization and specificity of three monoclonal antibodies against the 58-kilodalton protein of Legionella pneumophila.

Sampson J, Plikaytis B, Aloisio C, Carlone G, Pau C, Stinson A J Clin Microbiol. 1991; 29(4):836-41.

PMID: 1890189 PMC: 269885. DOI: 10.1128/jcm.29.4.836-841.1991.