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Purification, Partial Characterization, and Seroreactivity of a Genuswide 60-kilodalton Legionella Protein Antigen

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1988 Jan 1
PMID 3343316
Citations 8
Authors
C P Pau
B B Plikaytis
G M Carlone
I M Warner
Affiliations
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Abstract

A genuswide protein antigen extracted from Legionella pneumophila serogroup 1 (strain Philadelphia 1) cells was enriched by differential pelleting and ammonium sulfate precipitation and subsequently purified with a combination of high-performance size-exclusion and ion-exchange chromatography. The protein has an apparent molecular weight of 650,000 before and 63,000 after urea (5 M) treatment, as determined by size-exclusion chromatography. These proteins resolved to a single band of 60,000 after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The urea-treated protein had an isoelectric point of 5.8. This purified 60-kilodalton protein reacted with a convalescent-phase serum sample from a patient with legionellosis and rabbit immune sera prepared against each of 23 Legionella species. The 60-kilodalton protein may be useful in developing diagnostic tests for legionellosis.

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