Assessment of Lactic Acid Bacteria Isolated from the Chicken Digestive Tract for Potential Use As Poultry Probiotics

Overview

Journal Anim Biosci

Specialty Veterinary Medicine

Date 2023 May 12

PMID 37170515

Authors

Merisa Sirisopapong

Takeshi Shimosato

Supattra Okrathok

Sutisa Khempaka

Affiliations

Soon will be listed here.

Abstract

Objective: The use of probiotics as an alternative to antibiotics in animal feed has received considerable attention in recent decades. Lactic acid bacteria (LAB) have remarkable functional properties promoting host health and are major microorganisms for probiotic purposes. The aim of this study was to characterize LAB strains of the chicken digestive tract and to determine their functional properties for further use as potential probiotics in poultry.

Methods: A total of 2,000 colonies were isolated from the ileum and cecal contents of the chickens based on their phenotypic profiles and followed by a preliminary detection for acid and bile tolerance. The selected 200 LAB isolates with exhibited well-tolerance in acid and bile conditions were then identified by sequencing the 16S rDNA gene, followed by acid and bile tolerance, antimicrobial activity, adhesion to epithelial cells and additional characteristics on the removal of cholesterol. Then, the two probiotic strains (L. ingluviei and L. salivarious) which showed the greatest advantage in vitro testing were selected to assess their efficacy in broiler chickens.

Results: It was found that 200 LAB isolates that complied with all measurement criteria belonged to five strains, including L. acidophilus (63 colonies), L. ingluviei (2 colonies), L. reuteri (58 colonies), L. salivarius (72 colonies), and L. saerimneri (5 colonies). We found that the L. ingluviei and L. salivarius can increase the population of LAB and Bifidobacterium spp. while reducing Enterobacteria spp. and Escherichia coli in the cecal content of chickens. Additionally, increased concentrations of valeric acid and short chain fatty acids were also observed.

Conclusion: This study indicates that all five Lactobacillus strains isolated from gut contents of chickens are safe and possess probiotic properties, especially L. ingluviei and L. salivarius. Future studies should evaluate the potential for growth improvement in broilers.

Citing Articles

Probiotics in Poultry: Unlocking Productivity Through Microbiome Modulation and Gut Health.

Naeem M, Bourassa D Microorganisms. 2025; 13(2).

PMID: 40005624 PMC: 11857632. DOI: 10.3390/microorganisms13020257.

Oral administration of C37 inhibits colonization in chicks.

Murakami A, Watanabe-Yanai A, Iwata T, Namai F, Sato T, Fujii T Front Microbiol. 2024; 15:1491039.

PMID: 39669788 PMC: 11634846. DOI: 10.3389/fmicb.2024.1491039.

Hypothetical proteins of chicken-isolated Limosilactobacillus reuteri subjected to in silico analyses induce IL-2 and IL-10.

Adejumo I Genes Nutr. 2024; 19(1):21.

PMID: 39425027 PMC: 11490116. DOI: 10.1186/s12263-024-00755-4.

In vitro probiotic potential of lactic acid bacteria isolated from the intestines of Muscovy ducks.

de Carvalho Maquine L, Dos Santos Almeida Coelho K, da Silva Gomes M, Vieira J, Cavalcante T, de Souza Carvalho E Braz J Microbiol. 2024; 55(4):4115-4128.

PMID: 39080108 PMC: 11711415. DOI: 10.1007/s42770-024-01474-z.

Identification of novel probiotic lactic acid bacteria from soymilk waste using the 16s rRNA gene for potential use in poultry.

Srifani A, Mirnawati M, Marlida Y, Rizal Y, Nurmiati N, Lee K Vet World. 2024; 17(5):1001-1011.

PMID: 38911076 PMC: 11188893. DOI: 10.14202/vetworld.2024.1001-1011.

References

Aruwa C, Pillay C, Nyaga M, Sabiu S . Poultry gut health - microbiome functions, environmental impacts, microbiome engineering and advancements in characterization technologies. J Anim Sci Biotechnol. 2021; 12(1):119. PMC: 8638651. DOI: 10.1186/s40104-021-00640-9. View

Sureshkumar S, Lee H, Jung S, Kim D, Oh K, Yang H . Inclusion of Lactobacillus salivarius strain revealed a positive effect on improving growth performance, fecal microbiota and immunological responses in chicken. Arch Microbiol. 2020; 203(2):847-853. DOI: 10.1007/s00203-020-02088-3. View

Mountzouris K, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G, Fegeros K . Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult Sci. 2007; 86(2):309-17. DOI: 10.1093/ps/86.2.309. View

Erkkila S, Petaja E . Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use. Meat Sci. 2011; 55(3):297-300. DOI: 10.1016/s0309-1740(99)00156-4. View

Jose N, Bunt C, Hussain M . Comparison of Microbiological and Probiotic Characteristics of Lactobacilli Isolates from Dairy Food Products and Animal Rumen Contents. Microorganisms. 2016; 3(2):198-212. PMC: 5023236. DOI: 10.3390/microorganisms3020198. View

Monteagudo-Mera A, Rastall R, Gibson G, Charalampopoulos D, Chatzifragkou A . Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health. Appl Microbiol Biotechnol. 2019; 103(16):6463-6472. PMC: 6667406. DOI: 10.1007/s00253-019-09978-7. View

Noohi N, Papizadeh M, Rohani M, Talebi M, Pourshafie M . Screening for probiotic characters in lactobacilli isolated from chickens revealed the intra-species diversity of . Anim Nutr. 2021; 7(1):119-126. PMC: 8110883. DOI: 10.1016/j.aninu.2020.07.005. View

Onrust L, Van Driessche K, Ducatelle R, Schwarzer K, Haesebrouck F, Van Immerseel F . Valeric acid glyceride esters in feed promote broiler performance and reduce the incidence of necrotic enteritis. Poult Sci. 2018; 97(7):2303-2311. DOI: 10.3382/ps/pey085. View

Morelli L . In vitro selection of probiotic lactobacilli: a critical appraisal. Curr Issues Intest Microbiol. 2001; 1(2):59-67. View

10.

Dec M, Urban-Chmiel R, Stepien-Pysniak D, Wernicki A . Assessment of antibiotic susceptibility in isolates from chickens. Gut Pathog. 2017; 9:54. PMC: 5605976. DOI: 10.1186/s13099-017-0203-z. View

11.

Nami Y, Abdullah N, Haghshenas B, Radiah D, Rosli R, Khosroushahi A . Probiotic potential and biotherapeutic effects of newly isolated vaginal Lactobacillus acidophilus 36YL strain on cancer cells. Anaerobe. 2014; 28:29-36. DOI: 10.1016/j.anaerobe.2014.04.012. View

12.

Tsukagoshi M, Sirisopapong M, Namai F, Ishida M, Okrathok S, Shigemori S . Lactobacillus ingluviei C37 from chicken inhibits inflammation in LPS-stimulated mouse macrophages. Anim Sci J. 2020; 91(1):e13436. DOI: 10.1111/asj.13436. View

13.

Vieco-Saiz N, Belguesmia Y, Raspoet R, Auclair E, Gancel F, Kempf I . Benefits and Inputs From Lactic Acid Bacteria and Their Bacteriocins as Alternatives to Antibiotic Growth Promoters During Food-Animal Production. Front Microbiol. 2019; 10:57. PMC: 6378274. DOI: 10.3389/fmicb.2019.00057. View

14.

Kizerwetter-Swida M, Binek M . Assessment of potentially probiotic properties of Lactobacillus strains isolated from chickens. Pol J Vet Sci. 2016; 19(1):15-20. DOI: 10.1515/pjvs-2016-0003. View

15.

Pennacchia C, Vaughan E, Villani F . Potential probiotic Lactobacillus strains from fermented sausages: Further investigations on their probiotic properties. Meat Sci. 2011; 73(1):90-101. DOI: 10.1016/j.meatsci.2005.10.019. View

16.

Angelakis E, Bastelica D, Ben Amara A, El Filali A, Dutour A, Mege J . An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice. Microb Pathog. 2011; 52(1):61-8. DOI: 10.1016/j.micpath.2011.10.004. View

17.

Qing Y, Xie H, Su C, Wang Y, Yu Q, Pang Q . Gut Microbiome, Short-Chain Fatty Acids, and Mucosa Injury in Young Adults with Human Immunodeficiency Virus Infection. Dig Dis Sci. 2018; 64(7):1830-1843. DOI: 10.1007/s10620-018-5428-2. View

18.

Al-Khalaifa H, Al-Nasser A, Al-Surayee T, Al-Kandari S, Al-Enzi N, Al-Sharrah T . Effect of dietary probiotics and prebiotics on the performance of broiler chickens. Poult Sci. 2019; 98(10):4465-4479. DOI: 10.3382/ps/pez282. View

19.

Dowarah R, Verma A, Agarwal N, Singh P, Singh B . Selection and characterization of probiotic lactic acid bacteria and its impact on growth, nutrient digestibility, health and antioxidant status in weaned piglets. PLoS One. 2018; 13(3):e0192978. PMC: 5843174. DOI: 10.1371/journal.pone.0192978. View

20.

Mookiah S, Sieo C, Ramasamy K, Abdullah N, Wan Ho Y . Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J Sci Food Agric. 2013; 94(2):341-8. DOI: 10.1002/jsfa.6365. View