CBMs As Probes to Explore Plant Cell Wall Heterogeneity Using Immunocytochemistry

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 May 6

PMID 37149530

Authors

Louise Badruna

Vincent Burlat

Cedric Y Montanier

Affiliations

Soon will be listed here.

Abstract

Immunocytochemistry is a widely used technique to localize antigen within intact tissues. Plant cell walls are complex matrixes of highly decorated polysaccharides and the large number of CBM families displaying specific substrate recognition reflects this complexity. The accessibility of large proteins, such as antibodies, to their cell wall epitopes may be sometimes difficult due to steric hindrance problems. Due to their smaller size, CBMs are interesting alternative probes. The aim of this chapter is to describe the use of CBM as probes to explore complex polysaccharide topochemistry in muro and to quantify enzymatic deconstruction.

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