Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 May 4

PMID 37142928

Authors

Dominik Schneidereit

Stefanie Nubler

Oliver Friedrich

Affiliations

Soon will be listed here.

Abstract

The architectural structure of cells is essential for the cells' function, which becomes especially apparent in the highly "structure functionally" tuned skeletal muscle cells. Here, structural changes in the microstructure can have a direct impact on performance parameters, such as isometric or tetanic force production. The microarchitecture of the actin-myosin lattice in muscle cells can be detected noninvasively in living cells and in 3D by using second harmonic generation (SHG) microscopy, forgoing the need to alter samples by introducing fluorescent probes into them. Here, we provide tools and step-by-step protocols to guide the processes of obtaining SHG microscopy image data from samples, as well as extracting characteristic values from the image data to quantify the cellular microarchitecture using characteristic patterns of myofibrillar lattice alignments.

References

Berchtold M, Brinkmeier H, Muntener M . Calcium ion in skeletal muscle: its crucial role for muscle function, plasticity, and disease. Physiol Rev. 2000; 80(3):1215-65. DOI: 10.1152/physrev.2000.80.3.1215. View

Buttgereit A, Weber C, Friedrich O . A novel quantitative morphometry approach to assess regeneration in dystrophic skeletal muscle. Neuromuscul Disord. 2014; 24(7):596-603. DOI: 10.1016/j.nmd.2014.04.011. View

Buttgereit A, Weber C, Garbe C, Friedrich O . From chaos to split-ups--SHG microscopy reveals a specific remodelling mechanism in ageing dystrophic muscle. J Pathol. 2012; 229(3):477-85. DOI: 10.1002/path.4136. View

Centonze V, White J . Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys J. 1998; 75(4):2015-24. PMC: 1299873. DOI: 10.1016/S0006-3495(98)77643-X. View

Franzini-Armstrong C . Electron Microscopy: From 2D to 3D Images with Special Reference to Muscle. Eur J Transl Myol. 2016; 25(1):4836. PMC: 4748974. DOI: 10.4081/ejtm.2015.4836. View

Friedrich O, Both M, Weber C, Schurmann S, Teichmann M, von Wegner F . Microarchitecture is severely compromised but motor protein function is preserved in dystrophic mdx skeletal muscle. Biophys J. 2010; 98(4):606-16. PMC: 2820646. DOI: 10.1016/j.bpj.2009.11.005. View

Garbe C, Buttgereit A, Schurmann S, Friedrich O . Automated multiscale morphometry of muscle disease from second harmonic generation microscopy using tensor-based image processing. IEEE Trans Biomed Eng. 2011; 59(1):39-44. DOI: 10.1109/TBME.2011.2167325. View

Haug M, Ritter P, Michael M, Reischl B, Schurmann S, Prols G . Structure-Function Relationships in Muscle Fibres: MyoRobot Online Assessment of Muscle Fibre Elasticity and Sarcomere Length Distributions. IEEE Trans Biomed Eng. 2021; 69(1):148-155. DOI: 10.1109/TBME.2021.3089739. View

Liu W, Raben N, Ralston E . Quantitative evaluation of skeletal muscle defects in second harmonic generation images. J Biomed Opt. 2013; 18(2):26005. PMC: 3564230. DOI: 10.1117/1.JBO.18.2.026005. View

10.

Mohler W, Millard A, Campagnola P . Second harmonic generation imaging of endogenous structural proteins. Methods. 2003; 29(1):97-109. DOI: 10.1016/s1046-2023(02)00292-x. View

11.

Preibisch S, Saalfeld S, Tomancak P . Globally optimal stitching of tiled 3D microscopic image acquisitions. Bioinformatics. 2009; 25(11):1463-5. PMC: 2682522. DOI: 10.1093/bioinformatics/btp184. View

12.

Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T . Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012; 9(7):676-82. PMC: 3855844. DOI: 10.1038/nmeth.2019. View

13.

Schneidereit D, Brollochs A, Ritter P, Kreiss L, Mokhtari Z, Beilhack A . An advanced optical clearing protocol allows label-free detection of tissue necrosis multiphoton microscopy in injured whole muscle. Theranostics. 2021; 11(6):2876-2891. PMC: 7806485. DOI: 10.7150/thno.51558. View

14.

Schneidereit D, Nubler S, Prolss G, Reischl B, Schurmann S, Muller O . Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach. Light Sci Appl. 2018; 7:79. PMC: 6199289. DOI: 10.1038/s41377-018-0080-3. View

15.

Schurmann S, von Wegner F, H A Fink R, Friedrich O, Vogel M . Second harmonic generation microscopy probes different states of motor protein interaction in myofibrils. Biophys J. 2010; 99(6):1842-51. PMC: 2941014. DOI: 10.1016/j.bpj.2010.07.005. View