Standardization of Organoid Culture in Cancer Research

Overview

Journal Cancer Med

Specialty Oncology

Date 2023 Apr 21

PMID 37081739

Authors

Changchun Zhou

Yuanbo Wu

Zeyu Wang

Yanli Liu

Jiaqi Yu

Weiping Wang

Sunrui Chen

Weihua Wu

Jidong Wang

Guowei Qian

Aina He

Affiliations

Soon will be listed here.

Abstract

Establishing a valid in vitro model to represent tumor heterogeneity and biology is critical but challenging. Tumor organoids are self-assembled three-dimensional cell clusters which are of great significance for recapitulating the histopathological, genetic, and phenotypic characteristics of primary tissues. The organoid has emerged as an attractive in vitro platform for tumor biology research and high-throughput drug screening in cancer medicine. Organoids offer unique advantages over cell lines and patient-derived xenograft models, but there are no standardized methods to guide the culture of organoids, leading to confusion in organoid studies that may affect accurate judgments of tumor biology. This review summarizes the shortcomings of current organoid culture methods, presents the latest research findings on organoid standardization, and proposes an outlook for organoid modeling.

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