Isolation and Characterization of Brown Adipose Tissue T Cells

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Apr 19

PMID 37076683

Authors

Ling-Ran Kong

Cheng-Chao Ruan

Affiliations

Soon will be listed here.

Abstract

Brown adipose tissue (BAT) is a specialized fat depot that can dissipate energy through uncoupled respiration and thermogenesis. Various immune cells such as macrophages, eosinophils, type 2 innate lymphoid cells, and T lymphocytes were recently found to have an unexpected involvement in controlling the thermogenic activity of brown adipose tissue. Here, we describe a protocol for isolation and characterization of T cells from brown adipose tissue.

References

Sidossis L, Kajimura S . Brown and beige fat in humans: thermogenic adipocytes that control energy and glucose homeostasis. J Clin Invest. 2015; 125(2):478-86. PMC: 4319444. DOI: 10.1172/JCI78362. View

Kim S, Plutzky J . Brown Fat and Browning for the Treatment of Obesity and Related Metabolic Disorders. Diabetes Metab J. 2016; 40(1):12-21. PMC: 4768046. DOI: 10.4093/dmj.2016.40.1.12. View

Lee P, Bova R, Schofield L, Bryant W, Dieckmann W, Slattery A . Brown Adipose Tissue Exhibits a Glucose-Responsive Thermogenic Biorhythm in Humans. Cell Metab. 2016; 23(4):602-9. DOI: 10.1016/j.cmet.2016.02.007. View

Villarroya F, Cereijo R, Villarroya J, Giralt M . Brown adipose tissue as a secretory organ. Nat Rev Endocrinol. 2016; 13(1):26-35. DOI: 10.1038/nrendo.2016.136. View

Villarroya J, Cereijo R, Gavalda-Navarro A, Peyrou M, Giralt M, Villarroya F . New insights into the secretory functions of brown adipose tissue. J Endocrinol. 2019; 243(2):R19-R27. DOI: 10.1530/JOE-19-0295. View

Yang Y, Dong X, Ma W, Guan L, Wang Y, Huang X . Proliferation, Differentiation and Immunoregulatory Capacities of Brown and White Adipose-Derived Stem Cells from Young and Aged Mice. Int J Stem Cells. 2020; 13(2):246-256. PMC: 7378905. DOI: 10.15283/ijsc20019. View

Gentile P, Orlandi A, Scioli M, Di Pasquali C, Bocchini I, Cervelli V . Concise review: adipose-derived stromal vascular fraction cells and platelet-rich plasma: basic and clinical implications for tissue engineering therapies in regenerative surgery. Stem Cells Transl Med. 2012; 1(3):230-6. PMC: 3659840. DOI: 10.5966/sctm.2011-0054. View

Villarroya F, Cereijo R, Villarroya J, Gavalda-Navarro A, Giralt M . Toward an Understanding of How Immune Cells Control Brown and Beige Adipobiology. Cell Metab. 2018; 27(5):954-961. DOI: 10.1016/j.cmet.2018.04.006. View

Nguyen K, Qiu Y, Cui X, Goh Y, Mwangi J, David T . Alternatively activated macrophages produce catecholamines to sustain adaptive thermogenesis. Nature. 2011; 480(7375):104-8. PMC: 3371761. DOI: 10.1038/nature10653. View

10.

Rao R, Long J, White J, Svensson K, Lou J, Lokurkar I . Meteorin-like is a hormone that regulates immune-adipose interactions to increase beige fat thermogenesis. Cell. 2014; 157(6):1279-1291. PMC: 4131287. DOI: 10.1016/j.cell.2014.03.065. View

11.

Cho K, Morris D, DelProposto J, Geletka L, Zamarron B, Martinez-Santibanez G . An MHC II-dependent activation loop between adipose tissue macrophages and CD4+ T cells controls obesity-induced inflammation. Cell Rep. 2014; 9(2):605-17. PMC: 4252867. DOI: 10.1016/j.celrep.2014.09.004. View