The Anti-Biofilm Activity and Mechanism of Apigenin-7-O-Glucoside Against and

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2023 Apr 18

PMID 37070126

Authors

Ze-Jun Pei

Chengcheng Li

Wenna Dai

Zaixiang Lou

Xin Sun

Hongxin Wang

Azmat Ali Khan

Chunpeng Wan

Affiliations

Soon will be listed here.

Abstract

Introduction: This study aimed to examine the anti-biofilm activity and mechanism of gallic acid (GA), kaempferol-7-O-glucoside (K7G) and apigenin-7-O-glucoside (A7G) against and

Methods: The antibacterial activity of the natural compounds was determined by serial dilution method. The inhibitory activity of natural compounds on biofilms was determined by crystal violet staining method. The effects and mechanisms of natural compounds on bacterial biofilms were analyzed by atomic force microscopy.

Results: In our study, compared with GA and K7G, A7G was found to exhibit the strongest anti-biofilm and antibacterial activities. The minimum biofilm inhibitory concentration (MBIC) of A7G against and was 0.20 mg/mL and 0.10 mg/mL, respectively. The inhibition rates of 1/2 MIC of A7G on biofilms of and were 88.9%, and 83.2% respectively. Moreover, atomic force microscope (AFM) images showed the three-dimensional biofilm morphology of and , and the results indicated that A7G was highly effective in biofilm inhibition.

Discussion: It was found that the inhibition of A7G on biofilm was achieved through inhibiting on exopolysaccharides (EPS), quorum sensing (QS), and cell surface hydrophobicity (CSH). A7G exerted strong anti-biofilm activities by inhibiting EPS production, QS, and CSH. Hence, A7G, as a natural substance, could be a promising novel antibacterial and anti-biofilm agent for control of biofilm in food industry.

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