A Flexible and Versatile System for Multi-color Fiber Photometry and Optogenetic Manipulation

Overview

Journal Cell Rep Methods

Specialty Cell Biology

Date 2023 Apr 14

PMID 37056369

Authors

Andrey Formozov

Alexander Dieter

J Simon Wiegert

Affiliations

Soon will be listed here.

Abstract

Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.

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