Integrated Single-nucleus and Spatial Transcriptomics Captures Transitional States in Soybean Nodule Maturation

Overview

Journal Nat Plants

Specialties Biology
Genetics

Date 2023 Apr 13

PMID 37055554

Authors

Zhijian Liu

Xiangying Kong

Yanping Long

Sirui Liu

Hong Zhang

Jinbu Jia

Wenhui Cui

Zunmian Zhang

Xianwei Song

Lijuan Qiu

Jixian Zhai

Zhe Yan

Affiliations

Soon will be listed here.

Abstract

Legumes form symbiosis with rhizobium leading to the development of nitrogen-fixing nodules. By integrating single-nucleus and spatial transcriptomics, we established a cell atlas of soybean nodules and roots. In central infected zones of nodules, we found that uninfected cells specialize into functionally distinct subgroups during nodule development, and revealed a transitional subtype of infected cells with enriched nodulation-related genes. Overall, our results provide a single-cell perspective for understanding rhizobium-legume symbiosis.

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