In Vivo Mutagenicity Assessment of Styrene in MutaMouse Liver and Lung

Overview

Journal Genes Environ

Publisher Biomed Central

Specialty Environmental Health

Date 2023 Apr 11

PMID 37041654

Authors

Yasumasa Murata

Masakatsu Natsume

Takako Iso

Yoshiyuki Shigeta

Nozomu Hirose

Takaaki Umano

Katsuyoshi Horibata

Kei-Ichi Sugiyama

Kenichi Masumura

Akihiko Hirose

Mariko Matsumoto

Affiliations

Soon will be listed here.

Abstract

Background: Styrene (CAS 100-42-5) is widely used as polystyrene and acrylonitrile-butadiene-styrene resin such as plastic, rubber, and paint. One of the primary uses of styrene is food utensils and containers, but a small amount of styrene transferred into food can be ingested by eating. Styrene is metabolized into styrene 7,8-oxide (SO). SO is mutagenic in bacteria and mouse lymphoma assays. It is clastogenic in cultured mammalian cells. However, styrene and SO are not clastogenic/aneugenic in rodents, and no rodent in vivo gene mutation studies were identified.

Methods: To investigate the mutagenicity of orally administered styrene, we used the transgenic rodent gene mutation assay to perform an in vivo mutagenicity test (OECD TG488). The transgenic MutaMouse was given styrene orally at doses of 0 (corn oil; negative control), 75, 150, and 300 mg/kg/day for 28 days, and mutant frequencies (MFs) were determined using the lacZ assay in the liver and lung (five male mice/group).

Results: There were no significant differences in the MFs of the liver and lung up to 300 mg/kg/day (close to maximum tolerable dose (MTD)), when one animal with extremely high MFs that were attributed to an incidental clonal mutation was omitted. Positive and negative controls produced the expected results.

Conclusions: These findings show that styrene is not mutagenic in the liver and lung of MutaMouse under this experimental condition.

Citing Articles

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Lack of in vivo mutagenicity of carbendazim in the liver and glandular stomach of MutaMice.

Iso T, Suzuki K, Murata Y, Hirose N, Umano T, Horibata K Genes Environ. 2024; 46(1):7.

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