Immortalized Cell Lines Derived from Dental/odontogenic Tissue

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 2023 Apr 11

PMID 37039940

Authors

Yanglin Zeng

Liu Liu

Dingming Huang

Dongzhe Song

Affiliations

Soon will be listed here.

Abstract

Stem cells derived from dental/odontogenic tissue have the property of multiple differentiation and are prospective in tooth regenerative medicine and cellular and molecular studies. However, in the face of cellular senescence soon in vitro, the proliferation ability of the cells is limited, so studies are hindered to some extent. Fortunately, immortalization strategies are expected to solve the above issues. Cellular immortalization is that cells are immortalized by introducing oncogenes, human telomerase reverse transcriptase genes (hTERT), or miscellaneous immortalization genes to get unlimited proliferation. At present, a variety of immortalized stem cells from dental/odontogenic tissue has been successfully generated, such as dental pulp stem cells (DPSCs), periodontal ligament cells (PDLs), stem cells from human exfoliated deciduous teeth (SHEDs), dental papilla cells (DPCs), and tooth germ mesenchymal cells (TGMCs). This review summarized establishment and applications of immortalized stem cells from dental/odontogenic tissues and then discussed the advantages and challenges of immortalization.

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References

Alcorta D, Xiong Y, Phelps D, Hannon G, Beach D, Barrett J . Involvement of the cyclin-dependent kinase inhibitor p16 (INK4a) in replicative senescence of normal human fibroblasts. Proc Natl Acad Sci U S A. 1996; 93(24):13742-7. PMC: 19411. DOI: 10.1073/pnas.93.24.13742. View

Maciel Palacio Alvarez M, de Carvalho R, Barbosa S, Polassi M, Nascimento F, DAlpino P . Oxidative stress induced by self-adhesive resin cements affects gene expression, cellular proliferation and mineralization potential of the MDPC-23 odontoblast-like cells. Dent Mater. 2019; 35(4):606-616. DOI: 10.1016/j.dental.2019.02.008. View

Anitua E, Troya M, Zalduendo M . Progress in the use of dental pulp stem cells in regenerative medicine. Cytotherapy. 2018; 20(4):479-498. DOI: 10.1016/j.jcyt.2017.12.011. View

Aranha A, Giro E, Souza P, Hebling J, de Souza Costa C . Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line. Dent Mater. 2006; 22(9):864-9. DOI: 10.1016/j.dental.2005.11.015. View

Arany S, Kawagoe M, Sugiyama T . Application of spontaneously immortalized odontoblast cells in tooth regeneration. Biochem Biophys Res Commun. 2009; 381(1):84-9. DOI: 10.1016/j.bbrc.2009.02.025. View

Arany S, Nakata A, Kameda T, Koyota S, Ueno Y, Sugiyama T . Phenotype properties of a novel spontaneously immortalized odontoblast-lineage cell line. Biochem Biophys Res Commun. 2006; 342(3):718-24. DOI: 10.1016/j.bbrc.2006.02.020. View

Asakawa T, Yamada A, Kugino M, Hasegawa T, Yoshimura K, Sasa K . Establishment of Down's syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT. Hum Cell. 2021; 35(1):379-383. PMC: 8732922. DOI: 10.1007/s13577-021-00621-0. View

Beausejour C, Krtolica A, Galimi F, Narita M, Lowe S, Yaswen P . Reversal of human cellular senescence: roles of the p53 and p16 pathways. EMBO J. 2003; 22(16):4212-22. PMC: 175806. DOI: 10.1093/emboj/cdg417. View

Belgiovine C, Chiodi I, Mondello C . Telomerase: cellular immortalization and neoplastic transformation. Multiple functions of a multifaceted complex. Cytogenet Genome Res. 2009; 122(3-4):255-62. DOI: 10.1159/000167811. View

10.

Bozic D, Grgurevic L, Erjavec I, Brkljacic J, Orlic I, Razdorov G . The proteome and gene expression profile of cementoblastic cells treated by bone morphogenetic protein-7 in vitro. J Clin Periodontol. 2011; 39(1):80-90. DOI: 10.1111/j.1600-051X.2011.01794.x. View

11.

Cao Y, Tan Q, Li J, Wang J . Bone morphogenetic proteins 2, 6, and 9 differentially regulate the osteogenic differentiation of immortalized preodontoblasts. Braz J Med Biol Res. 2020; 53(9):e9750. PMC: 7413614. DOI: 10.1590/1414-431X20209750. View

12.

Chen J, Xu H, Xia K, Cheng S, Zhang Q . Resolvin E1 accelerates pulp repair by regulating inflammation and stimulating dentin regeneration in dental pulp stem cells. Stem Cell Res Ther. 2021; 12(1):75. PMC: 7821538. DOI: 10.1186/s13287-021-02141-y. View

13.

Chen L, Couwenhoven R, Hsu D, Luo W, Snead M . Maintenance of amelogenin gene expression by transformed epithelial cells of mouse enamel organ. Arch Oral Biol. 1992; 37(10):771-8. DOI: 10.1016/0003-9969(92)90110-t. View

14.

Chen W, Xie J, Lin X, Ou M, Zhou J, Wei X . The Role of Small Extracellular Vesicles Derived from Lipopolysaccharide-preconditioned Human Dental Pulp Stem Cells in Dental Pulp Regeneration. J Endod. 2021; 47(6):961-969. DOI: 10.1016/j.joen.2021.03.010. View

15.

Chen X, Wang Q, Gu K, Li A, Fu X, Wang Y . Effect of YAP on an Immortalized Periodontal Ligament Stem Cell Line. Stem Cells Int. 2019; 2019:6804036. PMC: 6466850. DOI: 10.1155/2019/6804036. View

16.

Chun Y, Foster B, Lukasavage P, Berry J, Zhao M, Tenenbaum H . Bisphosphonate modulates cementoblast behavior in vitro. J Periodontol. 2005; 76(11):1890-900. DOI: 10.1902/jop.2005.76.11.1890. View

17.

Colgin L, Reddel R . Telomere maintenance mechanisms and cellular immortalization. Curr Opin Genet Dev. 1999; 9(1):97-103. DOI: 10.1016/s0959-437x(99)80014-8. View

18.

Counter C, Hahn W, Wei W, Caddle S, Beijersbergen R, Lansdorp P . Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc Natl Acad Sci U S A. 1998; 95(25):14723-8. PMC: 24516. DOI: 10.1073/pnas.95.25.14723. View

19.

dAdda di Fagagna F, Reaper P, Clay-Farrace L, Fiegler H, Carr P, von Zglinicki T . A DNA damage checkpoint response in telomere-initiated senescence. Nature. 2003; 426(6963):194-8. DOI: 10.1038/nature02118. View

20.

DErrico J, Berry J, Ouyang H, Strayhorn C, Windle J, Somerman M . Employing a transgenic animal model to obtain cementoblasts in vitro. J Periodontol. 2000; 71(1):63-72. DOI: 10.1902/jop.2000.71.1.63. View