Duplex Real-time PCR Assay with High-resolution Melt Analysis for the Detection and Quantification of Species and in Meat Products

Overview

Journal J Food Sci Technol

Specialty Nutritional Sciences

Date 2023 Apr 10

PMID 37033312

Authors

M R Vishnuraj

G Ajay

N Aravind Kumar

J Renuka

Niveditha Pollumahanti

H Anusha Chauhan

S Vaithiyanathan

Deepak B Rawool

S B Barbuddhe

Affiliations

Soon will be listed here.

Abstract

Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-023-05695-2.

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References

Singh J, Batish V, Grover S . A molecular beacon-based duplex real-time polymerase chain reaction assay for simultaneous detection of Escherichia coli O157:H7 and Listeria monocytogenes in milk and milk products. Foodborne Pathog Dis. 2009; 6(10):1195-201. DOI: 10.1089/fpd.2009.0310. View

Grazina L, Costa J, Amaral J, Mafra I . High-Resolution Melting Analysis as a Tool for Plant Species Authentication. Methods Mol Biol. 2020; 2264:55-73. DOI: 10.1007/978-1-0716-1201-9_5. View

Barbau-Piednoir E, Botteldoorn N, Yde M, Mahillon J, Roosens N . Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes. Appl Microbiol Biotechnol. 2012; 97(9):4021-37. PMC: 3627020. DOI: 10.1007/s00253-012-4477-2. View

Carlin C, Liao J, Weller D, Guo X, Orsi R, Wiedmann M . Listeria cossartiae sp. nov., Listeria farberi sp. nov., Listeria immobilis sp. nov., Listeria portnoyi sp. nov. and Listeria rustica sp. nov., isolated from agricultural water and natural environments. Int J Syst Evol Microbiol. 2021; 71(5). PMC: 8289207. DOI: 10.1099/ijsem.0.004795. View

Tchatchouang C, Fri J, De Santi M, Brandi G, Schiavano G, Amagliani G . Listeriosis Outbreak in South Africa: A Comparative Analysis with Previously Reported Cases Worldwide. Microorganisms. 2020; 8(1). PMC: 7023107. DOI: 10.3390/microorganisms8010135. View

Lee H, Loh T, Lee C, Tang J, Chiu L, Koay E . A universal influenza A and B duplex real-time RT-PCR assay. J Med Virol. 2012; 84(10):1646-51. PMC: 7166972. DOI: 10.1002/jmv.23375. View

Gebretsadik S, Kassa T, Alemayehu H, Huruy K, Kebede N . Isolation and characterization of Listeria monocytogenes and other Listeria species in foods of animal origin in Addis Ababa, Ethiopia. J Infect Public Health. 2011; 4(1):22-9. DOI: 10.1016/j.jiph.2010.10.002. View

Kawasaki S, Horikoshi N, Okada Y, Takeshita K, Sameshima T, Kawamoto S . Multiplex PCR for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in meat samples. J Food Prot. 2005; 68(3):551-6. DOI: 10.4315/0362-028x-68.3.551. View

Vergis J, Negi M, Poharkar K, Das D, Malik S, Kumar A . 16S rRNA PCR followed by restriction endonuclease digestion: a rapid approach for genus level identification of important enteric bacterial pathogens. J Microbiol Methods. 2013; 95(3):353-6. DOI: 10.1016/j.mimet.2013.10.001. View

10.

Quereda J, Leclercq A, Moura A, Vales G, Gomez-Martin A, Garcia-Munoz A . sp. nov., isolated from a water trough and the faeces of healthy sheep. Int J Syst Evol Microbiol. 2020; 70(11):5868-5879. DOI: 10.1099/ijsem.0.004494. View

11.

Pietzka A, Allerberger F, Murer A, Lennkh A, Stoger A, Cabal Rosel A . Whole Genome Sequencing Based Surveillance of for Early Detection and Investigations of Listeriosis Outbreaks. Front Public Health. 2019; 7:139. PMC: 6557975. DOI: 10.3389/fpubh.2019.00139. View

12.

Rodriguez-Lazaro D, Hernandez M, Pla M . Simultaneous quantitative detection of Listeria spp. and Listeria monocytogenes using a duplex real-time PCR-based assay. FEMS Microbiol Lett. 2004; 233(2):257-67. DOI: 10.1016/j.femsle.2004.02.018. View

13.

Desai A, Anyoha A, Madoff L, Lassmann B . Changing epidemiology of Listeria monocytogenes outbreaks, sporadic cases, and recalls globally: A review of ProMED reports from 1996 to 2018. Int J Infect Dis. 2019; 84:48-53. PMC: 6579688. DOI: 10.1016/j.ijid.2019.04.021. View

14.

Liu Y, Zhao L, Song X, Du P, Li D, Chen Z . Development of fluorogenic probe-based and high-resolution melting-based polymerase chain reaction assays for the detection and differentiation of Bartonella quintana and Bartonella henselae. J Microbiol Methods. 2016; 138:30-36. DOI: 10.1016/j.mimet.2016.06.014. View

15.

Wachiralurpan S, Phung-On I, Chanlek N, Areekit S, Chansiri K, Lieberzeit P . Monitoring of Real-Time Loop-Mediated Isothermal Amplification with QCM: Detecting . Biosensors (Basel). 2021; 11(9). PMC: 8470657. DOI: 10.3390/bios11090308. View

16.

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

17.

Cremonesi P, Cortimiglia C, Picozzi C, Minozzi G, Malvisi M, Luini M . Development of a Droplet Digital Polymerase Chain Reaction for Rapid and Simultaneous Identification of Common Foodborne Pathogens in Soft Cheese. Front Microbiol. 2016; 7:1725. PMC: 5083709. DOI: 10.3389/fmicb.2016.01725. View

18.

Mackiw E, Korsak D, Kowalska J, Felix B, Stasiak M, Kucharek K . Genetic diversity of Listeria monocytogenes isolated from ready-to-eat food products in retail in Poland. Int J Food Microbiol. 2021; 358:109397. DOI: 10.1016/j.ijfoodmicro.2021.109397. View

19.

Raufu I, Moura A, Vales G, Ahmed O, Aremu A, Thouvenot P . sp. nov., isolated from cow milk cheese in Nigeria. Int J Syst Evol Microbiol. 2022; 72(6). DOI: 10.1099/ijsem.0.005437. View

20.

Amagliani G, Blasi G, Scuota S, Duranti A, Fisichella S, Gattuso A . Detection and Virulence Characterization of Strains in Ready-to-Eat Products. Foodborne Pathog Dis. 2021; 18(9):675-682. DOI: 10.1089/fpd.2020.2923. View