RopB Represses the Transcription of in the Absence of SIP in Group A

Overview

Journal Life Sci Alliance

Specialties Biochemistry
Biology
Cell Biology
Molecular Biology

Date 2023 Mar 31

PMID 37001914

Authors

Chuan Chiang-Ni

Yan-Wen Chen

Kai-Lin Chen

Jian-Xian Jiang

Yong-An Shi

Chih-Yun Hsu

Yi-Ywan M Chen

Chih-Ho Lai

Cheng-Hsun Chiu

Affiliations

Soon will be listed here.

Abstract

RopB is a quorum-sensing regulator that binds to the SpeB-inducing peptide (SIP) under acidic conditions. SIP is known to be degraded by the endopeptidase PepO, whose transcription is repressed by the CovR/CovS two-component regulatory system. Both SIP-bound RopB (RopB-SIP) and SIP-free RopB (apo-RopB) can bind to the promoter; however, only RopB-SIP activates transcription. In this study, we found that the SpeB expression was higher in the mutant than in the SIP-inactivated (*) mutant. Furthermore, the deletion of in the * mutant derepressed expression, suggesting that apo-RopB is a transcriptional repressor of Up-regulation of PepO in the mutant degraded SIP, resulting in the down-regulation of We demonstrate that deleting in the mutant derepressed the expression, suggesting that the repression in this mutant was mediated not only by PepO-dependent SIP degradation but also by apo-RopB. These findings reveal a crosstalk between the CovR/CovS and RopB-SIP systems and redefine the role of RopB in regulating expression in group A .

Citing Articles

RopB-regulated SpeB cysteine protease degrades extracellular vesicles-associated streptolysin O and bacterial proteins from group A .

Chiang-Ni C, Chiang C, Chen Y, Shi Y, Chao Y, Wang S Virulence. 2023; 14(1):2249784.

PMID: 37621107 PMC: 10461520. DOI: 10.1080/21505594.2023.2249784.

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