Facile Electrochemical Affinity Measurements of Small and Large Molecules

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2023 Mar 30

PMID 36994086

Authors

Pouya Zaree

Ilhan Tomris

Sander D de Vos

Roosmarijn van der Woude

Frits M Flesch

Robertus J M Klein Gebbink

Robert P de Vries

Roland J Pieters

Affiliations

Soon will be listed here.

Abstract

A novel miniaturized sensor for electrochemical detection that contains graphene- and gold nanoparticles was functionalized with proteins. Using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) it was possible to observe and quantify interactions of molecules with these proteins. The protein binders included carbohydrate ligands as small as carbohydrates up to COVID-19 spike protein variants engaged in protein-protein interactions. The system uses off-the-shelf sensors combined with an affordable potentiostat and yet is sensitive enough for small ligand binding.

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