The Role of the Preanalytical Step for Human Saliva Analysis Via Vibrational Spectroscopy

Overview

Journal Metabolites

Publisher MDPI

Date 2023 Mar 29

PMID 36984834

Authors

Beatrice Campanella

Stefano Legnaioli

Massimo Onor

Edoardo Benedetti

Emilia Bramanti

Affiliations

Soon will be listed here.

Abstract

Saliva is an easily sampled matrix containing a variety of biochemical information, which can be correlated with the individual health status. The fast, straightforward analysis of saliva by vibrational (ATR-FTIR and Raman) spectroscopy is a good premise for large-scale preclinical studies to aid translation into clinics. In this work, the effects of saliva collection (spitting/swab) and processing (two different deproteinization procedures) were explored by principal component analysis (PCA) of ATR-FTIR and Raman data and by investigating the effects on the main saliva metabolites by reversed-phase chromatography (RPC-HPLC-DAD). Our results show that, depending on the bioanalytical information needed, special care must be taken when saliva is collected with swabs because the polymeric material significantly interacts with some saliva components. Moreover, the analysis of saliva before and after deproteinization by FTIR and Raman spectroscopy allows to obtain complementary biological information.

Citing Articles

Advances in Metabolic Profiling of Biological Samples.

Pinto J Metabolites. 2023; 13(4).

PMID: 37110192 PMC: 10143415. DOI: 10.3390/metabo13040534.

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