Magnolol Loaded on Carboxymethyl Chitosan Particles Improved the Antimicrobial Resistance and Storability of Kiwifruits

Overview

Journal Foods

Specialty Biotechnology

Date 2023 Mar 29

PMID 36981076

Authors

Feixu Mo

Wenzhi Li

Youhua Long

Rongyu Li

Yi Ding

Ming Li

Affiliations

Soon will be listed here.

Abstract

Magnolol is a natural compound extracted from the traditional Chinese medicine , which exhibits antimicrobial properties. However, magnolol is insoluble in water and consists of a phenolic hydroxyl group, which is volatile; these factors hinder its application. In this study, a safe and environmentally friendly method to improve the microbial resistance and storability of harvested fruits is developed using the water-soluble carrier carboxymethyl chitosan (CMCS) and magnolol. Magnolol was loaded on CMCS particles to form Magnolol@CMCS antimicrobial particles, a preservation coating agent. Magnolol@CMCS particles effectively solved the problems of water insolubility and agglomeration of magnolol and reduced the size distribution D50 value of magnolol from 0.749 to 0.213 μm. Magnolol@CMCS particles showed greater toxicity against , , and than that of magnolol alone, with effective medium concentration (EC) values of 0.9408, 142.4144, and 8.8028 μg/mL, respectively. Kiwifruit treated with the Magnolol@CMCS solution showed delayed changes in fruit hardness and soluble solid and dry matter contents and significantly higher ascorbic acid (vitamin C) and soluble total sugar contents and sugar:acid ratios compared with that of the control fruit. In addition, no disease spots were observed on fruit treated with the Magnolol@CMCS solution within 7 days after inoculation with . In conclusion, Magnolol@CMCS particles showed antimicrobial activity on harvested fruits, effectively delayed the hardness and nutritional changes of fruits during storage, and improved the storability of kiwifruit.

Citing Articles

Polymer-Free Electrospinning of β-Cyclodextrin-Oligolactide for Magnolol and Honokiol Pharmaceutical Formulations.

Blaj D, Peptu C, Balan-Porcarasu M, Peptu C, Tuchilus C, Ochiuz L Pharmaceutics. 2025; 17(1.

PMID: 39861776 PMC: 11768894. DOI: 10.3390/pharmaceutics17010130.

Research progress on inhibitors and inhibitory mechanisms of mycotoxin biosynthesis.

Li M, Li H Mycotoxin Res. 2024; 40(4):483-494.

PMID: 39164466 DOI: 10.1007/s12550-024-00553-2.

Advances in Postharvest Preservation and Quality of Fruits and Vegetables.

Valenzuela J Foods. 2023; 12(9).

PMID: 37174367 PMC: 10178206. DOI: 10.3390/foods12091830.

References

Zheng H, Liu W, Yang S . Carboxymethyl chitosan coated medium-chain fatty acid nanoliposomes: structure, composition, stability and release investigation. Food Funct. 2021; 12(20):9947-9954. DOI: 10.1039/d1fo01985f. View

Kim J, Ro E, Yoon K . Comparison of Growth Kinetics of Various Pathogenic on Fresh Perilla Leaf. Foods. 2017; 2(3):364-373. PMC: 5302300. DOI: 10.3390/foods2030364. View

Song Y, Weng Z, Dou T, Finel M, Wang Y, Ding L . Inhibition of human carboxylesterases by magnolol: Kinetic analyses and mechanism. Chem Biol Interact. 2019; 308:339-349. DOI: 10.1016/j.cbi.2019.06.003. View

Zhou P, Fu J, Hua H, Liu X . In vitro inhibitory activities of magnolol against spp. Drug Des Devel Ther. 2017; 11:2653-2661. PMC: 5593404. DOI: 10.2147/DDDT.S146529. View

Kumar S, Mudai A, Roy B, Basumatary I, Mukherjee A, Dutta J . Biodegradable Hybrid Nanocomposite of Chitosan/Gelatin and Green Synthesized Zinc Oxide Nanoparticles for Food Packaging. Foods. 2020; 9(9). PMC: 7555077. DOI: 10.3390/foods9091143. View

Wu J, Xue Z, Miao J, Zhang F, Gao X, Liu X . Sensitivity of Different Developmental Stages and Resistance Risk Assessment of to Fluopicolide in China. Front Microbiol. 2020; 11:185. PMC: 7064020. DOI: 10.3389/fmicb.2020.00185. View

Friel E, Wang M, Taylor A, MacRae E . In vitro and in vivo release of aroma compounds from yellow-fleshed kiwifruit. J Agric Food Chem. 2007; 55(16):6664-73. DOI: 10.1021/jf063733x. View

Hou R, Wu J, Yang L, Zhao K, Huang S, Kaziem A . Preparation of alginate-chitosan floating granules loaded with 2-methyl-4-chlorophenoxy acetic acid (MCPA) and their bioactivity on water hyacinth. Pest Manag Sci. 2021; 77(9):3942-3951. DOI: 10.1002/ps.6414. View

Shi X, Fang Q, Ding M, Wu J, Ye F, Lv Z . Microspheres of carboxymethyl chitosan, sodium alginate and collagen for a novel hemostatic in vitro study. J Biomater Appl. 2015; 30(7):1092-102. DOI: 10.1177/0885328215618354. View

10.

Bartholomew H, Bradshaw M, Jurick 2nd W, Fonseca J . The Good, the Bad, and the Ugly: Mycotoxin Production During Postharvest Decay and Their Influence on Tritrophic Host-Pathogen-Microbe Interactions. Front Microbiol. 2021; 12:611881. PMC: 7907610. DOI: 10.3389/fmicb.2021.611881. View

11.

Song X, Liu L, Wu X, Liu Y, Yuan J . Chitosan-Based Functional Films Integrated with Magnolol: Characterization, Antioxidant and Antimicrobial Activity and Pork Preservation. Int J Mol Sci. 2021; 22(15). PMC: 8345937. DOI: 10.3390/ijms22157769. View

12.

Zhou J, Hu Y, Chen P, Zhang H . Preparation of restricted access monolithic tip via unidirectional freezing and atom transfer radical polymerization for directly extracting magnolol and honokiol from rat plasma followed by liquid chromatography analysis. J Chromatogr A. 2020; 1625:461238. DOI: 10.1016/j.chroma.2020.461238. View

13.

Du Y, Yang F, Yu H, Cheng Y, Guo Y, Yao W . Fabrication of novel self-healing edible coating for fruits preservation and its performance maintenance mechanism. Food Chem. 2021; 351:129284. DOI: 10.1016/j.foodchem.2021.129284. View

14.

Huang Y, Sun C, Guan X, Lian S, Li B, Wang C . Butylated Hydroxytoluene Induced Resistance Against in Apple Fruit. Front Microbiol. 2021; 11:599062. PMC: 7840594. DOI: 10.3389/fmicb.2020.599062. View

15.

Lin Y, Li N, Lin H, Lin M, Chen Y, Wang H . Effects of chitosan treatment on the storability and quality properties of longan fruit during storage. Food Chem. 2019; 306:125627. DOI: 10.1016/j.foodchem.2019.125627. View

16.

Gocheva Y, Krumova E, Slokoska L, Miteva J, Vassilev S, Angelova M . Cell response of Antarctic and temperate strains of Penicillium spp. to different growth temperature. Mycol Res. 2006; 110(Pt 11):1347-54. DOI: 10.1016/j.mycres.2006.08.007. View

17.

Choi E, Kim H, Kim J, Jun S, Kang S, Park D . The herbal-derived honokiol and magnolol enhances immune response to infection with methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA). Appl Microbiol Biotechnol. 2015; 99(10):4387-96. DOI: 10.1007/s00253-015-6382-y. View

18.

Saito J, Sakai Y, Nagase H . In vitro anti-mutagenic effect of magnolol against direct and indirect mutagens. Mutat Res. 2006; 609(1):68-73. DOI: 10.1016/j.mrgentox.2006.06.021. View

19.

Cao J, Yan J, Zhao Y, Jiang W . Effects of postharvest salicylic acid dipping on Alternaria rot and disease resistance of jujube fruit during storage. J Sci Food Agric. 2013; 93(13):3252-8. DOI: 10.1002/jsfa.6167. View

20.

Wang L, Wang D, Yuan S, Feng X, Wang M . Transcriptomic Insights into the Antifungal Effects of Magnolol on the Growth and Mycotoxin Production of . Toxins (Basel). 2020; 12(10). PMC: 7594048. DOI: 10.3390/toxins12100665. View