PDZK1-Interacting Protein 1(PDZKIP1) Inhibits Goat Subcutaneous Preadipocyte Differentiation Through Promoting Autophagy

Overview

Journal Animals (Basel)

Date 2023 Mar 29

PMID 36978587

Authors

Dingshuang Chen

Yanyan Li

Tingting Hu

Chengsi Gong

Guangyu Lu

Xiaotong Ma

Yong Wang

Youli Wang

Yaqiu Lin

Affiliations

Soon will be listed here.

Abstract

PDZK1IP1 is highly expressed in tumor tissue and has been identified as a tumor biomarker. However, the role of PDZK1IP1 in goat subcutaneous preadipocyte differentiation remains largely unknown. The molecular mechanism of autophagy in regulating the differentiation of goat subcutaneous preadipocytes has not been clarified yet. In our study, PDZK1IP1 gain of function and loss of function were performed to reveal its functions in preadipocyte differentiation and autophagy. Our results showed that the overexpression of PDZK1IP1 inhibited the differentiation of goat subcutaneous preadipocytes, whereas it promoted autophagy. Consistently, the knockdown of PDZK1IP1 demonstrated the opposite tendency. Next, we investigated whether PDZK1IP1 inhibited the differentiation of goat preadipocytes by regulating autophagy. We found that inhibiting autophagy can rescue the PDZK1IP1-induced differentiation restraint in goat subcutaneous preadipocytes. In conclusion, PDZK1IP1 acts as a regulator of adipogenesis, and inhibits goat subcutaneous preadipocyte differentiation through promoting autophagy. Our results will contribute to further understanding the role and mechanism of PDZK1IP1 in controlling adipogenesis.

Citing Articles

FOS Inhibits the Differentiation of Intramuscular Adipocytes in Goats.

Hu T, Li Z, Gong C, Xiong Y, Sun S, Xing J Genes (Basel). 2023; 14(11).

PMID: 38003034 PMC: 10671551. DOI: 10.3390/genes14112088.

References

Nicklas B, Penninx B, Cesari M, Kritchevsky S, Newman A, Kanaya A . Association of visceral adipose tissue with incident myocardial infarction in older men and women: the Health, Aging and Body Composition Study. Am J Epidemiol. 2004; 160(8):741-9. DOI: 10.1093/aje/kwh281. View

Zhang W, Li P, Wang S, Cheng G, Wang L, Mi X . TP53INP2 Promotes Bovine Adipocytes Differentiation Through Autophagy Activation. Animals (Basel). 2019; 9(12). PMC: 6940805. DOI: 10.3390/ani9121060. View

Sandeep S, Gokulakrishnan K, Velmurugan K, Deepa M, Mohan V . Visceral & subcutaneous abdominal fat in relation to insulin resistance & metabolic syndrome in non-diabetic south Indians. Indian J Med Res. 2010; 131:629-35. View

Kessel D, Price M, Reiners Jr J . ATG7 deficiency suppresses apoptosis and cell death induced by lysosomal photodamage. Autophagy. 2012; 8(9):1333-41. PMC: 3442880. DOI: 10.4161/auto.20792. View

S Mesquita F, Thomas M, Sachse M, Santos A, Figueira R, Holden D . The Salmonella deubiquitinase SseL inhibits selective autophagy of cytosolic aggregates. PLoS Pathog. 2012; 8(6):e1002743. PMC: 3375275. DOI: 10.1371/journal.ppat.1002743. View

Schumacher M, DelCurto-Wyffels H, Thomson J, Boles J . Fat Deposition and Fat Effects on Meat Quality-A Review. Animals (Basel). 2022; 12(12). PMC: 9219498. DOI: 10.3390/ani12121550. View

Furukawa K, Innokentev A, Kanki T . Mitophagy regulation mediated by the Far complex in yeast. Autophagy. 2021; 17(4):1042-1043. PMC: 8078710. DOI: 10.1080/15548627.2021.1885184. View

Li X, Zhang H, Wang Y, Li Y, He C, Zhu J . RNA-seq analysis reveals the positive role of KLF5 in the differentiation of subcutaneous adipocyte in goats. Gene. 2021; 808:145969. DOI: 10.1016/j.gene.2021.145969. View

Yeh W, Bierer B, McKnight S . Rapamycin inhibits clonal expansion and adipogenic differentiation of 3T3-L1 cells. Proc Natl Acad Sci U S A. 1995; 92(24):11086-90. PMC: 40576. DOI: 10.1073/pnas.92.24.11086. View

10.

Guijarro M, Castro M, Romero L, Moneo V, Carnero A . Large scale genetic screen identifies MAP17 as protein bypassing TNF-induced growth arrest. J Cell Biochem. 2007; 101(1):112-21. DOI: 10.1002/jcb.21163. View

11.

Chen D, Wang R, Sheng X, Zhao N, Lin Y, Wang Y . PDZK1-interacting protein 1(PDZK1IP1) promotes subcutaneous preadipocyte proliferation in goats. Anim Biotechnol. 2022; 34(7):3063-3073. DOI: 10.1080/10495398.2022.2132952. View

12.

Kocher O, Cheresh P, Lee S . Identification and partial characterization of a novel membrane-associated protein (MAP17) up-regulated in human carcinomas and modulating cell replication and tumor growth. Am J Pathol. 1996; 149(2):493-500. PMC: 1865305. View

13.

Yu K, Lu H, Chen Y, Xin Y, Tan Z, Yang Q . 80MAP17 promotes the tumorigenesis of papillary thyroid carcinoma by reducing the stability of p53. Front Biosci (Landmark Ed). 2021; 26(10):777-788. DOI: 10.52586/4987. View

14.

Jaeger C, Schaefer B, Wallich R, Kramer M . The membrane-associated protein pKe#192/MAP17 in human keratinocytes. J Invest Dermatol. 2000; 115(3):375-80. DOI: 10.1046/j.1523-1747.2000.00071.x. View

15.

Yoshii S, Mizushima N . Monitoring and Measuring Autophagy. Int J Mol Sci. 2017; 18(9). PMC: 5618514. DOI: 10.3390/ijms18091865. View

16.

Garcia-Heredia J, Carnero A . Dr. Jekyll and Mr. Hyde: MAP17's up-regulation, a crosspoint in cancer and inflammatory diseases. Mol Cancer. 2018; 17(1):80. PMC: 5896160. DOI: 10.1186/s12943-018-0828-7. View

17.

Mizushima N, Ohsumi Y, Yoshimori T . Autophagosome formation in mammalian cells. Cell Struct Funct. 2003; 27(6):421-9. DOI: 10.1247/csf.27.421. View

18.

Trayhurn P, Beattie J . Physiological role of adipose tissue: white adipose tissue as an endocrine and secretory organ. Proc Nutr Soc. 2001; 60(3):329-39. DOI: 10.1079/pns200194. View

19.

Galluzzi L, Green D . Autophagy-Independent Functions of the Autophagy Machinery. Cell. 2019; 177(7):1682-1699. PMC: 7173070. DOI: 10.1016/j.cell.2019.05.026. View

20.

Kovsan J, Bluher M, Tarnovscki T, Kloting N, Kirshtein B, Madar L . Altered autophagy in human adipose tissues in obesity. J Clin Endocrinol Metab. 2010; 96(2):E268-77. DOI: 10.1210/jc.2010-1681. View