Research Overview of L-DOPA Production Using a Bacterial Enzyme, Tyrosine Phenol-lyase

Overview

Journal Proc Jpn Acad Ser B Phys Biol Sci

Specialties Biology
Science

Date 2023 Mar 13

PMID 36908174

Authors

Hidehiko Kumagai

Takane Katayama

Takashi Koyanagi

Hideyuki Suzuki

Affiliations

Soon will be listed here.

Abstract

L-DOPA is an amino acid that is used as a treatment for Parkinson's disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression.

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