Structural and Functional Characterization of the Cytotoxic Protein Ledodin, an Atypical Ribosome-inactivating Protein from Shiitake Mushroom (Lentinula Edodes)

Overview

Journal Protein Sci

Specialty Biochemistry

Date 2023 Mar 11

PMID 36905289

Authors

Lucia Citores

Sara Ragucci

Rosita Russo

Claudia C Gay

Angela Chambery

Antimo Di Maro

Rosario Iglesias

Jose M Ferreras

Affiliations

Soon will be listed here.

Abstract

We have purified ledodin, a cytotoxic 22-kDa protein from shiitake mushroom (Lentinula edodes) consisting of a 197 amino acid chain. Ledodin possessed N-glycosylase activity on the sarcin-ricin loop of mammalian 28S rRNA and inhibited protein synthesis. However, it was not active against insect, fungal, and bacterial ribosomes. In vitro and in silico studies suggested that ledodin exhibits a catalytic mechanism like that of DNA glycosylases and plant ribosome-inactivating proteins. Moreover, the sequence and structure of ledodin was not related to any protein of known function, although ledodin-homologous sequences were found in the genome of several species of fungi, some edible, belonging to different orders of the class Agaricomycetes. Therefore, ledodin could be the first of a new family of enzymes widely distributed among this class of basidiomycetes. The interest of these proteins lies both, in the fact that they can be a toxic agent of some edible mushrooms and in their application in medicine and biotechnology.

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