Effects and Mechanism of the Callus Culture Extract on Blue Light Damage in Human Foreskin Fibroblasts

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Mar 11

PMID 36903418

Authors

Xianyao Meng

Miaomiao Guo

Zaijun Geng

Ziqiang Wang

Huirong Zhang

Sunhua Li

Xiao Ling

Li Li

Affiliations

Soon will be listed here.

Abstract

is an important source of raw material for food, medicine, and modern cosmetics. The purpose of this study was to develop a new application for protection against blue light damage. To investigate the effects and mechanism of action of callus culture extract (LACCE) on blue light damage, a blue-light-induced human foreskin fibroblast damage model was established. The contents of collagen (COL-I), matrix metalloproteinase 1 (MMP-1), and opsin 3 (OPN3) were detected using enzyme-linked immunosorbent assays and Western blotting. The calcium influx and reactive oxygen species (ROS) levels were measured via flow cytometry and the results showed that the LACCE (10-15 mg/mL) promoted the production of COL-I, inhibited the secretion of MMP-1, OPN3, ROS and calcium influx, and may play a role in inhibiting the activation of blue light on the OPN3-calcium pathway. Thereafter, high-performance liquid chromatography and ultra-performance liquid chromatography-tandem mass spectrometry were used to quantitatively analyze the contents of nine active ingredients in the LACCE. The results indicated that LACCE has an anti-blue-light-damage effect and provides theoretical support for the development of new raw materials in the natural food, medicine, and skin care industries.

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References

Campiche R, Curpen S, Lutchmanen-Kolanthan V, Gougeon S, Cherel M, Laurent G . Pigmentation effects of blue light irradiation on skin and how to protect against them. Int J Cosmet Sci. 2020; 42(4):399-406. PMC: 7496068. DOI: 10.1111/ics.12637. View

Algvere P, Marshall J, Seregard S . Age-related maculopathy and the impact of blue light hazard. Acta Ophthalmol Scand. 2006; 84(1):4-15. DOI: 10.1111/j.1600-0420.2005.00627.x. View

Suh S, Choi E, Atanaskova Mesinkovska N . The expression of opsins in the human skin and its implications for photobiomodulation: A Systematic Review. Photodermatol Photoimmunol Photomed. 2020; 36(5):329-338. PMC: 7674233. DOI: 10.1111/phpp.12578. View

Austin E, Geisler A, Nguyen J, Kohli I, Hamzavi I, Lim H . Visible light. Part I: Properties and cutaneous effects of visible light. J Am Acad Dermatol. 2021; 84(5):1219-1231. PMC: 8887026. DOI: 10.1016/j.jaad.2021.02.048. View

Pralea I, Moldovan R, Tigu A, Petrache A, Heghes S, Mitoi M . Profiling of Polyphenolic Compounds of Cass Callus Cultures Using UPLC/IM-HRMS and Screening of In Vitro Effects. Plants (Basel). 2022; 11(1). PMC: 8747157. DOI: 10.3390/plants11010100. View

Reisinger U, Schwaiger S, Zeller I, Messner B, Stigler R, Wiedemann D . Leoligin, the major lignan from Edelweiss, inhibits intimal hyperplasia of venous bypass grafts. Cardiovasc Res. 2009; 82(3):542-9. PMC: 2682615. DOI: 10.1093/cvr/cvp059. View

Avola R, Graziano A, Pannuzzo G, Bonina F, Cardile V . Hydroxytyrosol from olive fruits prevents blue-light-induced damage in human keratinocytes and fibroblasts. J Cell Physiol. 2018; 234(6):9065-9076. DOI: 10.1002/jcp.27584. View

Olinski L, Lin E, Oancea E . Illuminating insights into opsin 3 function in the skin. Adv Biol Regul. 2019; 75:100668. PMC: 7059126. DOI: 10.1016/j.jbior.2019.100668. View

Marlot L, Batteau M, Escofet M, Nuccio S, Coquoin V, de Vaumas R . Two-dimensional multi-heart cutting centrifugal partition chromatography-liquid chromatography for the preparative isolation of antioxidants from Edelweiss plant. J Chromatogr A. 2017; 1504:55-63. DOI: 10.1016/j.chroma.2017.04.056. View

10.

Speroni E, Schwaiger S, Egger P, Berger A, Cervellati R, Govoni P . In vivo efficacy of different extracts of Edelweiss (Leontopodium alpinum Cass.) in animal models. J Ethnopharmacol. 2006; 105(3):421-6. DOI: 10.1016/j.jep.2005.11.019. View

11.

Mann T, Eggers K, Rippke F, Tesch M, Buerger A, Darvin M . High-energy visible light at ambient doses and intensities induces oxidative stress of skin-Protective effects of the antioxidant and Nrf2 inducer Licochalcone A in vitro and in vivo. Photodermatol Photoimmunol Photomed. 2019; 36(2):135-144. PMC: 7078816. DOI: 10.1111/phpp.12523. View

12.

Austin E, Huang A, Adar T, Wang E, Jagdeo J . Electronic device generated light increases reactive oxygen species in human fibroblasts. Lasers Surg Med. 2018; . DOI: 10.1002/lsm.22794. View

13.

Castellano-Pellicena I, Uzunbajakava N, Mignon C, Raafs B, Botchkarev V, Thornton M . Does blue light restore human epidermal barrier function via activation of Opsin during cutaneous wound healing?. Lasers Surg Med. 2018; 51(4):370-382. DOI: 10.1002/lsm.23015. View

14.

Lee R, Ko H, Kim K, Sohn Y, Min S, Kim J . Anti-melanogenic effects of extracellular vesicles derived from plant leaves and stems in mouse melanoma cells and human healthy skin. J Extracell Vesicles. 2020; 9(1):1703480. PMC: 6968621. DOI: 10.1080/20013078.2019.1703480. View

15.

Oplander C, Hidding S, Werners F, Born M, Pallua N, Suschek C . Effects of blue light irradiation on human dermal fibroblasts. J Photochem Photobiol B. 2011; 103(2):118-25. DOI: 10.1016/j.jphotobiol.2011.02.018. View

16.

Daniela L, Alla P, Maurelli R, Elena D, Giovanna P, Vladimir K . Anti-inflammatory effects of concentrated ethanol extracts of Edelweiss (Leontopodium alpinum Cass.) callus cultures towards human keratinocytes and endothelial cells. Mediators Inflamm. 2012; 2012:498373. PMC: 3474292. DOI: 10.1155/2012/498373. View

17.

Navarrete de Galvez E, Aguilera J, Solis A, de Galvez M, de Andres J, Herrera-Ceballos E . The potential role of UV and blue light from the sun, artificial lighting, and electronic devices in melanogenesis and oxidative stress. J Photochem Photobiol B. 2022; 228:112405. DOI: 10.1016/j.jphotobiol.2022.112405. View

18.

Arjmandi N, Mortazavi G, Zarei S, Faraz M, Mortazavi S . Can Light Emitted from Smartphone Screens and Taking Selfies Cause Premature Aging and Wrinkles?. J Biomed Phys Eng. 2018; 8(4):447-452. PMC: 6280109. View

19.

Dobner M, Sosa S, Schwaiger S, Altinier G, Della Loggia R, Kaneider N . Anti-inflammatory activity of Leontopodium alpinum and its constituents. Planta Med. 2004; 70(6):502-8. DOI: 10.1055/s-2004-827148. View

20.

Lan Y, Wang Y, Lu H . Opsin 3 is a key regulator of ultraviolet A-induced photoageing in human dermal fibroblast cells. Br J Dermatol. 2019; 182(5):1228-1244. PMC: 7318274. DOI: 10.1111/bjd.18410. View