Fun30 Nucleosome Remodeller Regulates White-to-opaque Switching in

Overview

Journal Acta Biochim Biophys Sin (Shanghai)

Specialties Biochemistry
Biophysics

Date 2023 Mar 10

PMID 36896644

Authors

Ning Gao

Baodi Dai

Xinyi Nie

Qun Zhao

Wencheng Zhu

Jiangye Chen

Affiliations

Soon will be listed here.

Abstract

( . ) is an opportunistic pathogen in humans and possesses a white-opaque heritable switching system. Wor1 is a master regulator of white-opaque switching and is essential for opaque cell formation in . . However, the regulatory network of Wor1 in white-opaque switching is still vague. In this study, we obtain a series of Wor1-interacting proteins using LexA-Wor1 as bait. Among these proteins, function unknown now 30 (Fun30) interacts with Wor1 and . Fun30 expression is upregulated in opaque cells at the transcriptional and protein levels. Loss of attenuates white-to-opaque switching, while ectopic expression of significantly increases white-to-opaque switching in an ATPase activity-dependent manner. Furthermore, upregulation is dependent on CO ; loss of , a key CO -sensing transcriptional regulator, abolishes upregulation. Interestingly, deletion of affects the expression regulation feedback loop. Thus, our results indicate that the chromatin remodeller Fun30 interacts with Wor1 and is required for expression and opaque cell formation.

References

Maurya P, Garai P, Goel K, Bhatt H, Dutta A, Goyal A . Fun30 and Rtt109 Mediate Epigenetic Regulation of the DNA Damage Response Pathway in . J Fungi (Basel). 2022; 8(6). PMC: 9225650. DOI: 10.3390/jof8060559. View

Du H, Guan G, Xie J, Cottier F, Sun Y, Jia W . The transcription factor Flo8 mediates CO2 sensing in the human fungal pathogen Candida albicans. Mol Biol Cell. 2012; 23(14):2692-701. PMC: 3395658. DOI: 10.1091/mbc.E12-02-0094. View

Huang G, Srikantha T, Sahni N, Yi S, Soll D . CO(2) regulates white-to-opaque switching in Candida albicans. Curr Biol. 2009; 19(4):330-4. PMC: 2667266. DOI: 10.1016/j.cub.2009.01.018. View

Fonzi W, Irwin M . Isogenic strain construction and gene mapping in Candida albicans. Genetics. 1993; 134(3):717-28. PMC: 1205510. DOI: 10.1093/genetics/134.3.717. View

Noble S, Gianetti B, Witchley J . Candida albicans cell-type switching and functional plasticity in the mammalian host. Nat Rev Microbiol. 2016; 15(2):96-108. PMC: 5957277. DOI: 10.1038/nrmicro.2016.157. View

Dai B, Xu Y, Wu H, Chen J . Rim101-upregulated Fets contribute to dark pigment formation in gray cells of Candida albicans. Acta Biochim Biophys Sin (Shanghai). 2021; 53(12):1723-1730. DOI: 10.1093/abbs/gmab142. View

Slutsky B, Buffo J, Soll D . High-frequency switching of colony morphology in Candida albicans. Science. 1985; 230(4726):666-9. DOI: 10.1126/science.3901258. View

Lohse M, Zordan R, Cain C, Johnson A . Distinct class of DNA-binding domains is exemplified by a master regulator of phenotypic switching in Candida albicans. Proc Natl Acad Sci U S A. 2010; 107(32):14105-10. PMC: 2922561. DOI: 10.1073/pnas.1005911107. View

Tong Z, Ai H, Li J . The Mechanism of Chromatin Remodeler SMARCAD1/Fun30 in Response to DNA Damage. Front Cell Dev Biol. 2020; 8:560098. PMC: 7545370. DOI: 10.3389/fcell.2020.560098. View

10.

Mao X, Cao F, Nie X, Liu H, Chen J . The Swi/Snf chromatin remodeling complex is essential for hyphal development in Candida albicans. FEBS Lett. 2006; 580(11):2615-22. DOI: 10.1016/j.febslet.2006.04.009. View

11.

Flaus A, Martin D, Barton G, Owen-Hughes T . Identification of multiple distinct Snf2 subfamilies with conserved structural motifs. Nucleic Acids Res. 2006; 34(10):2887-905. PMC: 1474054. DOI: 10.1093/nar/gkl295. View

12.

Lin A, Du Y, Xiao W . Yeast chromatin remodeling complexes and their roles in transcription. Curr Genet. 2020; 66(4):657-670. DOI: 10.1007/s00294-020-01072-0. View

13.

Srikantha T, Soll D . A white-specific gene in the white-opaque switching system of Candida albicans. Gene. 1993; 131(1):53-60. DOI: 10.1016/0378-1119(93)90668-s. View

14.

Ni J, Gao Y, Liu H, Chen J . Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production. FEBS Lett. 2004; 561(1-3):223-30. DOI: 10.1016/S0014-5793(04)00172-3. View

15.

Huang G, Yi S, Sahni N, Daniels K, Srikantha T, Soll D . N-acetylglucosamine induces white to opaque switching, a mating prerequisite in Candida albicans. PLoS Pathog. 2010; 6(3):e1000806. PMC: 2837409. DOI: 10.1371/journal.ppat.1000806. View

16.

Costelloe T, Louge R, Tomimatsu N, Mukherjee B, Martini E, Khadaroo B . The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection. Nature. 2012; 489(7417):581-4. PMC: 3493121. DOI: 10.1038/nature11353. View

17.

Lu Y, Su C, Mao X, Raniga P, Liu H, Chen J . Efg1-mediated recruitment of NuA4 to promoters is required for hypha-specific Swi/Snf binding and activation in Candida albicans. Mol Biol Cell. 2008; 19(10):4260-72. PMC: 2555938. DOI: 10.1091/mbc.e08-02-0173. View

18.

Kadosh D . Regulatory mechanisms controlling morphology and pathogenesis in Candida albicans. Curr Opin Microbiol. 2019; 52:27-34. PMC: 6874724. DOI: 10.1016/j.mib.2019.04.005. View

19.

Clark M, Zhong W, Keng T, Storms R, Barton A, Kaback D . Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I. Yeast. 1992; 8(2):133-45. DOI: 10.1002/yea.320080208. View

20.

Morrow B, Srikantha T, Anderson J, Soll D . Coordinate regulation of two opaque-phase-specific genes during white-opaque switching in Candida albicans. Infect Immun. 1993; 61(5):1823-8. PMC: 280771. DOI: 10.1128/iai.61.5.1823-1828.1993. View