Identification of and Splicing Variants in 5' Untranslated Region with Distinct Expression Profiles in Brain Tumor Samples

Overview

Journal Front Oncol

Specialty Oncology

Date 2023 Mar 2

PMID 36860313

Authors

Reihane Kazerani

Pouya Salehipour

Mohammadreza Shah Mohammadi

Elnaz Amanzadeh Jajin

Mohammad Hossein Modarressi

Affiliations

Soon will be listed here.

Abstract

Introduction: Brain tumors (BTs) are perceived as one of the most common malignancies among children. The specific regulation of each gene can play a critical role in cancer progression. The present study aimed to determine the transcripts of the and genes, considering the alternative 5'UTR region, and investigating the expression of these different transcripts in BTs.

Material And Methods: Public data on brain tumor microarray datasets in GEO were analyzed with R software to evaluate the expression levels of and genes (the Pheatmap package in R was also used to plot DEGs in a heat map). In addition, to validate our in-silico data analysis, RT-PCR was performed to determine the splicing variants of and genes in testis and brain tumor samples. The expression levels of splice variants of these genes were analyzed in 30 brain tumor samples and two testicular tissue samples as a positive control.

Results: In silico results show that the differential expression levels of and were significant in the GEO datasets of BTs compared to normal samples (with adjusted p-value<0.05 and log fold change > 1). This study's experimental results showed that the gene produces four different transcripts with two distinct promoter regions and splicing exon 4. The relative mRNA expression of transcripts without exon 4 was higher than transcripts with exon 4 in BT samples (p-value<001). In , exon 2 in the 5'UTR region and exon 6 in the coding sequence were spliced. The expression analysis results showed that the relative mRNA expression of transcript variants without exon 2 was higher than other transcript variants with exon 2 in BT samples (p-value<001).

Conclusion: The decreased expression levels of transcripts with longer 5'UTR in BT samples than in testicular or low-grade brain tumor samples may decrease their translation efficiency. Therefore, decreased amounts of TSGA10 and GGNBP2 as potential tumor suppressor proteins, especially in high-grade brain tumors, may cause cancer development by angiogenesis and metastasis.

Citing Articles

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Exploring TSGA10 Function: A Crosstalk or Controlling Mechanism in the Signaling Pathway of Carcinogenesis?.

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