Polymorphism Analysis of Interleukin-18 and Interleukin-37 Genes in Hepatitis B Infections with Different Outcomes: A Preliminary Report from an Iranian Population

Overview

Journal Asian Pac J Cancer Prev

Specialty Oncology

Date 2023 Feb 28

PMID 36853287

Authors

Vahid Molaei

Mohammad Reza Fattahi

Mohammad Reza Haghshenas

Seyed Younes Hosseini

Seyed Ali Malekhosseini

Jamal Sarvari

Affiliations

Soon will be listed here.

Abstract

Objective: Given the vital role of cytokines in influencing the outcomes of hepatitis B virus (HBV) infections, this study aimed to investigate the association between polymorphisms of interleukin (IL)-18 and IL-37 and the outcomes of HBV infection.

Methods: In this study, we enrolled 300 subjects with chronic HBV infection, including those with cirrhosis/hepatocellular carcinoma (C/HCC), chronic active hepatitis B (CAH) infection, or asymptomatic carriers (AC), and 58 individuals whose infection was spontaneously cleared (SC). Genomic DNA was extracted, and IL-18/IL-37 genotyping was performed using PCR-RFLP and ARMS-PCR.

Results: The frequency of genotypes and alleles of IL-18 single nucleotide polymorphisms (SNPs) at positions rs1946519, rs1946518, and rs187238 and IL-37 at position rs4241122 were not statistically different among the four studied groups (P>0.05). Furthermore, the frequency of different haplotypes was similar among the studied groups (P>0.05).

Conclusions: Polymorphisms of IL-18 SNPs at positions rs1946519, rs1946518, and rs187238 and variation of IL-37 at position rs4241122 do not appear to influence the outcome of HBV infection.

References

Gracie J, Koyama N, Murdoch J, Field M, McGarry F, Crilly A . Disease association of two distinct interleukin-18 promoter polymorphisms in Caucasian rheumatoid arthritis patients. Genes Immun. 2005; 6(3):211-6. DOI: 10.1038/sj.gene.6364183. View

Lu Y, Bao J, Deng Y, Rong C, Liu Y, Huang X . Role of IL-18 Gene Promoter Polymorphisms, Serum IL-18 Levels, and Risk of Hepatitis B Virus-related Liver Disease in the Guangxi Zhuang Population: a Retrospective Case-Control Study. Asian Pac J Cancer Prev. 2015; 16(14):6019-26. DOI: 10.7314/apjcp.2015.16.14.6019. View

Yang Y, Liu H . Association between interleukin-18 gene promoter (- 607C/A and - 137G/C) polymorphisms and chronic hepatitis C virus infections: A meta-analysis. Meta Gene. 2015; 5:21-31. PMC: 4443429. DOI: 10.1016/j.mgene.2015.04.004. View

Mahoney F . Update on diagnosis, management, and prevention of hepatitis B virus infection. Clin Microbiol Rev. 1999; 12(2):351-66. PMC: 88921. DOI: 10.1128/CMR.12.2.351. View

Song G, Choi S, Ji J, Lee Y . Association between interleukin-18 polymorphisms and systemic lupus erythematosus: a meta-analysis. Mol Biol Rep. 2012; 40(3):2581-7. DOI: 10.1007/s11033-012-2344-y. View

McInnes I, Gracie J, Leung B, Wei X, Liew F . Interleukin 18: a pleiotropic participant in chronic inflammation. Immunol Today. 2000; 21(7):312-5. DOI: 10.1016/s0167-5699(00)01648-0. View

Trepo C, Chan H, Lok A . Hepatitis B virus infection. Lancet. 2014; 384(9959):2053-63. DOI: 10.1016/S0140-6736(14)60220-8. View

Hosseini S, Sanaei N, Fattahi M, Malek-Hosseini S, Sarvari J . Association of HBsAg mutation patterns with hepatitis B infection outcome: Asymptomatic carriers versus HCC/cirrhotic patients. Ann Hepatol. 2019; 18(4):640-645. DOI: 10.1016/j.aohep.2018.12.006. View

Gheshlaghi A, Haghshenas M, Safarpour A, Hosseini S, Fattahi M, Sarvari J . IL-17 Genetic Variations Increase The Risk of Cirrhotic/Hepatocellular Carcinoma in Patients with Hepatitis B Virus Infection. Iran J Immunol. 2021; 18(2):130-140. DOI: 10.22034/iji.2021.88020.1852. View

10.

Yasuda K, Nakanishi K, Tsutsui H . Interleukin-18 in Health and Disease. Int J Mol Sci. 2019; 20(3). PMC: 6387150. DOI: 10.3390/ijms20030649. View

11.

Allam G, A Mohamed I, Alswat K, Abbadi S, Nassif R, Alharthi B . Association of IL-37 gene polymorphisms with susceptibility to tuberculosis in Saudi subjects. Microbiol Immunol. 2016; 60(11):778-786. DOI: 10.1111/1348-0421.12444. View

12.

Barbier L, Ferhat M, Salame E, Robin A, Herbelin A, Gombert J . Interleukin-1 Family Cytokines: Keystones in Liver Inflammatory Diseases. Front Immunol. 2019; 10:2014. PMC: 6718562. DOI: 10.3389/fimmu.2019.02014. View

13.

Liu H, Zheng R, Wang P, Yang H, He X, Ji Q . IL-37 Confers Protection against Mycobacterial Infection Involving Suppressing Inflammation and Modulating T Cell Activation. PLoS One. 2017; 12(1):e0169922. PMC: 5226736. DOI: 10.1371/journal.pone.0169922. View

14.

Zhu S, Zhao Y, Hu X, Luo T, Chen Z, Zhang Y . Genetic polymorphisms -137 (rs187238) and -607 (rs1946518) in the interleukin-18 promoter may not be associated with development of hepatocellular carcinoma. Sci Rep. 2016; 6:39404. PMC: 5175127. DOI: 10.1038/srep39404. View

15.

Liaw Y, Chu C . Hepatitis B virus infection. Lancet. 2009; 373(9663):582-92. DOI: 10.1016/S0140-6736(09)60207-5. View

16.

Bao J, Lu Y, Deng Y, Rong C, Liu Y, Huang X . Association between IL-18 polymorphisms, serum levels, and HBV-related hepatocellular carcinoma in a Chinese population: a retrospective case-control study. Cancer Cell Int. 2015; 15:72. PMC: 4513629. DOI: 10.1186/s12935-015-0223-z. View

17.

Ding V, Zhu Z, Mantz A, Xiao H, Wakefield M, Bai Q . The Role of IL-37 in Non-Cancerous Diseases. Pathol Oncol Res. 2016; 23(3):463-470. DOI: 10.1007/s12253-016-0137-7. View

18.

Ward J, Hinman A . What Is Needed to Eliminate Hepatitis B Virus and Hepatitis C Virus as Global Health Threats. Gastroenterology. 2018; 156(2):297-310. DOI: 10.1053/j.gastro.2018.10.048. View

19.

Zhou T, Chen Y, Hao L, Zhang Y . DC-SIGN and immunoregulation. Cell Mol Immunol. 2006; 3(4):279-83. View

20.

Hirankarn N, Manonom C, Tangkijvanich P, Poovorawan Y . Association of interleukin-18 gene polymorphism (-607A/A genotype) with susceptibility to chronic hepatitis B virus infection. Tissue Antigens. 2007; 70(2):160-3. DOI: 10.1111/j.1399-0039.2007.00865.x. View