Virgin Coconut Oil Reverses Behavioral Phenotypes of Letrozole-model of PCOS in Wistar Rats Via Modulation of NRF2 Upregulation

Overview

Journal J Taibah Univ Med Sci

Specialty General Medicine

Date 2023 Feb 28

PMID 36852244

Authors

Olabode O Akintoye

Ayodeji J Ajibare

Idowu O Omotuyi

Affiliations

Soon will be listed here.

Abstract

Objectives: Polycystic ovarian syndrome (PCOS) is an endocrine disorder associated with insulin resistance, hyperandrogenism, and sub-infertility. Virgin coconut oil (VCO) has been reported to have health benefits, such as anti-inflammatory, anti-oxidant, and antiviral properties. This study investigated the effects of dietary VCO supplementation on memory and cognitive impairment in female rats with letrozole induced PCOS.

Methods: Thirty female rats were randomly divided into five groups. All rats except controls were treated with letrozole for 21 days to induce PCOS and were subsequently treated for 14 days with 10% VCO, clomiphene (CLO), or VCO + CLO. Three neurobehavioral tests were conducted: elevated plus maze, Y maze, and novel object recognition tests.

Results: Our results indicated statistically elevated serum concentrations of sex hormones in rats with PCOS, compared with the control and treated groups. In addition, all treated groups showed significant reversal of the low serum concentrations of catalase and down-regulated gene expression of Nrf2 in the hippocampus seen in the PCOS rats. In addition, gene expression of acetylcholine esterase was up-regulated in PCOS rats, and was statistically reverted in the VCO treated groups.

Conclusion: Anxiety-like behavior and impaired short-term memory were observed in PCOS rats; however, VCO supplementation reversed these effects by modulating the gene expression of Nrf2 and AchE.

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References

Aled Rees D, Udiawar M, Berlot R, Jones D, OSullivan M . White Matter Microstructure and Cognitive Function in Young Women With Polycystic Ovary Syndrome. J Clin Endocrinol Metab. 2015; 101(1):314-23. PMC: 4701841. DOI: 10.1210/jc.2015-2318. View

Kleinridders A, Konner A, Bruning J . CNS-targets in control of energy and glucose homeostasis. Curr Opin Pharmacol. 2009; 9(6):794-804. DOI: 10.1016/j.coph.2009.10.006. View

Ghasemi S, Moradzadeh M, Hosseini M, Beheshti F, Sadeghnia H . Beneficial effects of Urtica dioica on scopolamine-induced memory impairment in rats: protection against acetylcholinesterase activity and neuronal oxidative damage. Drug Chem Toxicol. 2018; 42(2):167-175. DOI: 10.1080/01480545.2018.1463238. View

Katulski K, Podfigurna A, Czyzyk A, Meczekalski B, Genazzani A . Kisspeptin and LH pulsatile temporal coupling in PCOS patients. Endocrine. 2018; 61(1):149-157. PMC: 5997113. DOI: 10.1007/s12020-018-1609-1. View

Ghani N, Channip A, Chok Hwee Hwa P, Jaafar F, Yasin H, Usman A . Physicochemical properties, antioxidant capacities, and metal contents of virgin coconut oil produced by wet and dry processes. Food Sci Nutr. 2018; 6(5):1298-1306. PMC: 6060898. DOI: 10.1002/fsn3.671. View

Yan J, Zhang H, Yin Y, Li J, Tang Y, Purkayastha S . Obesity- and aging-induced excess of central transforming growth factor-β potentiates diabetic development via an RNA stress response. Nat Med. 2014; 20(9):1001-8. PMC: 4167789. DOI: 10.1038/nm.3616. View

Famurewa A, Folawiyo A, Enohnyaket E, Azubuike-Osu S, Abi I, Obaje S . Beneficial role of virgin coconut oil supplementation against acute methotrexate chemotherapy-induced oxidative toxicity and inflammation in rats. Integr Med Res. 2018; 7(3):257-263. PMC: 6160495. DOI: 10.1016/j.imr.2018.05.001. View

Marina A, Che Man Y, Nazimah S, Amin I . Antioxidant capacity and phenolic acids of virgin coconut oil. Int J Food Sci Nutr. 2008; 60 Suppl 2:114-23. DOI: 10.1080/09637480802549127. View

Cotter J, Muhlert N, Talwar A, Granger K . Examining the effectiveness of acetylcholinesterase inhibitors and stimulant-based medications for cognitive dysfunction in multiple sclerosis: A systematic review and meta-analysis. Neurosci Biobehav Rev. 2018; 86:99-107. DOI: 10.1016/j.neubiorev.2018.01.006. View

10.

Kuscu G, Gurel C, Buhur A, Oltulu F, Akman L, Kose T . The regulatory effects of clomiphene and tamoxifen on mTOR and LC3-II expressions in relation to autophagy in experimental polycystic ovary syndrome (PCOS). Mol Biol Rep. 2021; 49(3):1721-1729. DOI: 10.1007/s11033-021-06981-y. View

11.

Mao Y, Guo Z, Zheng T, Jiang Y, Yan Y, Yin X . Intranasal insulin alleviates cognitive deficits and amyloid pathology in young adult APPswe/PS1dE9 mice. Aging Cell. 2016; 15(5):893-902. PMC: 5013027. DOI: 10.1111/acel.12498. View

12.

Ostadmohammadi V, Jamilian M, Bahmani F, Asemi Z . Vitamin D and probiotic co-supplementation affects mental health, hormonal, inflammatory and oxidative stress parameters in women with polycystic ovary syndrome. J Ovarian Res. 2019; 12(1):5. PMC: 6340184. DOI: 10.1186/s13048-019-0480-x. View

13.

Barlampa D, Bompoula M, Bargiota A, Kalantaridou S, Mastorakos G, Valsamakis G . Hypothalamic Inflammation as a Potential Pathophysiologic Basis for the Heterogeneity of Clinical, Hormonal, and Metabolic Presentation in PCOS. Nutrients. 2021; 13(2). PMC: 7915850. DOI: 10.3390/nu13020520. View

14.

Rodriguez-Paris D, Remlinger-Molenda A, Kurzawa R, Glowinska A, Spaczynski R, Rybakowski F . Psychiatric disorders in women with polycystic ovary syndrome. Psychiatr Pol. 2019; 53(4):955-966. DOI: 10.12740/PP/OnlineFirst/93105. View

15.

Omotuyi O, Nash O, Enejoh O, Oribamise E, Adelakun N . flavonoids attenuate experimental nephropathy: Involvement of pro-inflammatory genes downregulation. Toxicol Rep. 2020; 7:1421-1427. PMC: 7578532. DOI: 10.1016/j.toxrep.2020.10.006. View

16.

Zhao F, Siu J, Huang W, Askwith C, Cao L . Insulin Modulates Excitatory Synaptic Transmission and Synaptic Plasticity in the Mouse Hippocampus. Neuroscience. 2019; 411:237-254. PMC: 6612444. DOI: 10.1016/j.neuroscience.2019.05.033. View

17.

Otuechere C, Madarikan G, Simisola T, Bankole O, Osho A . Virgin coconut oil protects against liver damage in albino rats challenged with the anti-folate combination, trimethoprim-sulfamethoxazole. J Basic Clin Physiol Pharmacol. 2013; 25(2):249-53. DOI: 10.1515/jbcpp-2013-0059. View

18.

Schliebs R, Arendt T . The cholinergic system in aging and neuronal degeneration. Behav Brain Res. 2010; 221(2):555-63. DOI: 10.1016/j.bbr.2010.11.058. View

19.

Shukla P, Mukherjee S . Mitochondrial dysfunction: An emerging link in the pathophysiology of polycystic ovary syndrome. Mitochondrion. 2020; 52:24-39. DOI: 10.1016/j.mito.2020.02.006. View

20.

Yang L, Qi Y, Yang Y . Astrocytes control food intake by inhibiting AGRP neuron activity via adenosine A1 receptors. Cell Rep. 2015; 11(5):798-807. DOI: 10.1016/j.celrep.2015.04.002. View