Strigolactones Modulate Stem Length and Diameter of Cherry Rootstocks Through Interaction with Other Hormone Signaling Pathways

Overview

Journal Front Plant Sci

Date 2023 Feb 27

PMID 36844087

Authors

Xunju Liu

Yan Xu

Wanxia Sun

Jiyuan Wang

Yixin Gao

Lei Wang

Wenping Xu

Shiping Wang

Songtao Jiu

Caixi Zhang

Affiliations

Soon will be listed here.

Abstract

Stem growth and development has considerable effects on plant architecture and yield performance. Strigolactones (SLs) modulate shoot branching and root architecture in plants. However, the molecular mechanisms underlying SLs regulate cherry rootstocks stem growth and development remain unclear. Our studies showed that the synthetic SL analog rac-GR24 and the biosynthetic inhibitor TIS108 affected stem length and diameter, aboveground weight, and chlorophyll content. The stem length of cherry rootstocks following TIS108 treatment reached a maximum value of 6.97 cm, which was much higher than that following rac-GR24 treatments at 30 days after treatment. Stem paraffin section showed that SLs affected cell size. A total of 1936, 743, and 1656 differentially expressed genes (DEGs) were observed in stems treated with 10 μM rac-GR24, 0.1 μM rac-GR24, and 10 μM TIS108, respectively. RNA-seq results highlighted several DEGs, including , , , , and , which play vital roles in stem growth and development. UPLC-3Q-MS analysis revealed that SL analogs and inhibitors affected the levels of several hormones in the stems. The endogenous GA content of stems increased significantly with 0.1 μM rac-GR24 or 10 μM TIS108 treatment, which is consistent with changes in the stem length following the same treatments. This study demonstrated that SLs affected stem growth of cherry rootstocks by changing other endogenous hormone levels. These results provide a solid theoretical basis for using SLs to modulate plant height and achieve sweet cherry dwarfing and high-density cultivation.

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References

Kende , van der Knaap E , CHO . Deepwater rice: A model plant to study stem elongation . Plant Physiol. 1998; 118(4):1105-10. PMC: 1539197. DOI: 10.1104/pp.118.4.1105. View

Wang L, Wang B, Yu H, Guo H, Lin T, Kou L . Transcriptional regulation of strigolactone signalling in Arabidopsis. Nature. 2020; 583(7815):277-281. DOI: 10.1038/s41586-020-2382-x. View

Agusti J, Herold S, Schwarz M, Sanchez P, Ljung K, Dun E . Strigolactone signaling is required for auxin-dependent stimulation of secondary growth in plants. Proc Natl Acad Sci U S A. 2011; 108(50):20242-7. PMC: 3250165. DOI: 10.1073/pnas.1111902108. View

Yu G, Wang L, Han Y, He Q . clusterProfiler: an R package for comparing biological themes among gene clusters. OMICS. 2012; 16(5):284-7. PMC: 3339379. DOI: 10.1089/omi.2011.0118. View

Screpanti C, Fonne-Pfister R, Lumbroso A, Rendine S, Lachia M, De Mesmaeker A . Strigolactone derivatives for potential crop enhancement applications. Bioorg Med Chem Lett. 2016; 26(10):2392-2400. DOI: 10.1016/j.bmcl.2016.03.072. View

Xu J, Zha M, Li Y, Ding Y, Chen L, Ding C . The interaction between nitrogen availability and auxin, cytokinin, and strigolactone in the control of shoot branching in rice (Oryza sativa L.). Plant Cell Rep. 2015; 34(9):1647-62. DOI: 10.1007/s00299-015-1815-8. View

Marzec M, Muszynska A . In silico analysis of the genes encoding proteins that are involved in the biosynthesis of the RMS/MAX/D pathway revealed new roles of Strigolactones in plants. Int J Mol Sci. 2015; 16(4):6757-82. PMC: 4424986. DOI: 10.3390/ijms16046757. View

Rial C, Varela R, Molinillo J, Lopez-Raez J, Macias F . A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts. Phytochem Anal. 2018; 30(1):110-116. DOI: 10.1002/pca.2796. View

Hayaloglu A, Demir N . Phenolic Compounds, Volatiles, and Sensory Characteristics of Twelve Sweet Cherry (Prunus avium L.) Cultivars Grown in Turkey. J Food Sci. 2015; 81(1):C7-18. DOI: 10.1111/1750-3841.13175. View

10.

Marzec M . Strigolactones and Gibberellins: A New Couple in the Phytohormone World?. Trends Plant Sci. 2017; 22(10):813-815. DOI: 10.1016/j.tplants.2017.08.001. View

11.

Xu Y, Wang J, Wang R, Wang L, Zhang C, Xu W . The Role of Strigolactones in the Regulation of Root System Architecture in Grapevine ( L.) in Response to Root-Restriction Cultivation. Int J Mol Sci. 2021; 22(16). PMC: 8395845. DOI: 10.3390/ijms22168799. View

12.

Jia H, Jiu S, Zhang C, Wang C, Tariq P, Liu Z . Abscisic acid and sucrose regulate tomato and strawberry fruit ripening through the abscisic acid-stress-ripening transcription factor. Plant Biotechnol J. 2016; 14(10):2045-65. PMC: 5043491. DOI: 10.1111/pbi.12563. View

13.

Zhang N, Xie Y, Guo H, Zhao L, Xiong H, Gu J . Gibberellins regulate the stem elongation rate without affecting the mature plant height of a quick development mutant of winter wheat (Triticum aestivum L.). Plant Physiol Biochem. 2016; 107:228-236. DOI: 10.1016/j.plaphy.2016.06.008. View

14.

Barbier F, Dun E, Kerr S, Chabikwa T, Beveridge C . An Update on the Signals Controlling Shoot Branching. Trends Plant Sci. 2019; 24(3):220-236. DOI: 10.1016/j.tplants.2018.12.001. View

15.

Ikeda M, Fujiwara S, Mitsuda N, Ohme-Takagi M . A triantagonistic basic helix-loop-helix system regulates cell elongation in Arabidopsis. Plant Cell. 2012; 24(11):4483-97. PMC: 3531847. DOI: 10.1105/tpc.112.105023. View

16.

Zou X, Wang Q, Chen P, Yin C, Lin Y . Strigolactones regulate shoot elongation by mediating gibberellin metabolism and signaling in rice (Oryza sativa L.). J Plant Physiol. 2019; 237:72-79. DOI: 10.1016/j.jplph.2019.04.003. View

17.

Raskin I, Kende H . Role of gibberellin in the growth response of submerged deep water rice. Plant Physiol. 1984; 76(4):947-50. PMC: 1064412. DOI: 10.1104/pp.76.4.947. View

18.

Liu X, Feng Z, Zhou C, Ren Y, Mou C, Wu T . Brassinosteroid (BR) biosynthetic gene lhdd10 controls late heading and plant height in rice (Oryza sativa L.). Plant Cell Rep. 2015; 35(2):357-68. DOI: 10.1007/s00299-015-1889-3. View

19.

Ito S, Yamagami D, Umehara M, Hanada A, Yoshida S, Sasaki Y . Regulation of Strigolactone Biosynthesis by Gibberellin Signaling. Plant Physiol. 2017; 174(2):1250-1259. PMC: 5462043. DOI: 10.1104/pp.17.00301. View

20.

Gallavotti A . The role of auxin in shaping shoot architecture. J Exp Bot. 2013; 64(9):2593-608. DOI: 10.1093/jxb/ert141. View