Application of Proteomics to Determine the Mechanism of Ozone on Sweet Cherries ( L.) by Time-series Analysis

Overview

Journal Front Plant Sci

Date 2023 Feb 27

PMID 36844069

Authors

Yuehan Zhao

Zhaohua Hou

Na Zhang

Haipeng Ji

Chenghu Dong

Jinze Yu

Xueling Chen

Cunkun Chen

Honglian Guo

Affiliations

Soon will be listed here.

Abstract

This research investigated the mechanism of ozone treatment on sweet cherry ( L.) by Lable-free quantification proteomics and physiological traits. The results showed that 4557 master proteins were identified in all the samples, and 3149 proteins were common to all groups. Mfuzz analyses revealed 3149 candidate proteins. KEGG annotation and enrichment analysis showed proteins related to carbohydrate and energy metabolism, protein, amino acids, and nucleotide sugar biosynthesis and degradation, and fruit parameters were characterized and quantified. The conclusions were supported by the fact that the qRT-PCR results agreed with the proteomics results. For the first time, this study reveals the mechanism of cherry in response to ozone treatment at a proteome level.

Citing Articles

Occurrence and Characterization of Causing Fruit Rot on Sweet Cherry in Southern China.

Ruan R, Huang K, Luo H, Zhang C, Xi D, Pei J Plants (Basel). 2023; 12(24).

PMID: 38140492 PMC: 10747181. DOI: 10.3390/plants12244165.

References

Yang S, Meng Z, Li Y, Chen R, Yang Y, Zhao Z . Evaluation of Physiological Characteristics, Soluble Sugars, Organic Acids and Volatile Compounds in 'Orin' Apples () at Different Ripening Stages. Molecules. 2021; 26(4). PMC: 7913981. DOI: 10.3390/molecules26040807. View

Li Y, Wang J, Wang K, Lyu S, Ren L, Huang C . Comparison analysis of widely-targeted metabolomics revealed the variation of potential astringent ingredients and their dynamic accumulation in the seed coats of both Carya cathayensis and Carya illinoinensis. Food Chem. 2021; 374:131688. DOI: 10.1016/j.foodchem.2021.131688. View

Cramer F, Englisch U, Freist W, Sternbach H . Aminoacylation of tRNAs as critical step of protein biosynthesis. Biochimie. 1991; 73(7-8):1027-35. DOI: 10.1016/0300-9084(91)90144-p. View

Mekontso F, Duan W, Cisse E, Chen T, Xu X . Alleviation of Postharvest Chilling Injury of Carambola Fruit by γ-aminobutyric Acid: Physiological, Biochemical, and Structural Characterization. Front Nutr. 2021; 8:752583. PMC: 8637291. DOI: 10.3389/fnut.2021.752583. View

Zheng G, Li W . Profiling membrane glycerolipids during γ-ray-induced membrane injury. BMC Plant Biol. 2017; 17(1):203. PMC: 5688707. DOI: 10.1186/s12870-017-1153-9. View

Wang S, Li W, Zhang X, Li G, Li X, Chang H . Metabolomics Study of Different Germplasm Resources for Three Species Using UPLC-Q-TOF-MS/MS. Front Plant Sci. 2022; 13:826902. PMC: 8963481. DOI: 10.3389/fpls.2022.826902. View

Kumar L, Futschik M . Mfuzz: a software package for soft clustering of microarray data. Bioinformation. 2007; 2(1):5-7. PMC: 2139991. DOI: 10.6026/97320630002005. View

Huang X, Hou Z . Label-free quantitative proteomics analysis of jujube ( Mill.) during different growth stages. RSC Adv. 2022; 11(36):22106-22119. PMC: 9034241. DOI: 10.1039/d1ra02989d. View

Huang D, Wang S, Song D, Cao X, Huang W, Ke S . Discovery of γ-Lactam Alkaloid Derivatives as Potential Fungicidal Agents Targeting Steroid Biosynthesis. J Agric Food Chem. 2020; 68(49):14438-14451. DOI: 10.1021/acs.jafc.0c05823. View

10.

Kim H . Lipid Metabolism in Plants. Plants (Basel). 2020; 9(7). PMC: 7411677. DOI: 10.3390/plants9070871. View

11.

Quiroga J, Alarcon P, Manosalva C, Teuber S, Carretta M, Burgos R . d-lactate-triggered extracellular trap formation in cattle polymorphonuclear leucocytes is glucose metabolism dependent. Dev Comp Immunol. 2022; 135:104492. DOI: 10.1016/j.dci.2022.104492. View

12.

Gao C, Lin Q, Dong C, Ji H, Yu J, Chen C . Effects of ozone concentration on the postharvest quality and microbial diversity of Muscat Hamburg grapes. RSC Adv. 2022; 10(15):9037-9045. PMC: 9050027. DOI: 10.1039/c9ra10479h. View

13.

Qin F, Lin L, Jia Y, Li W, Yu B . Quantitative Profiling of Arabidopsis Polar Glycerolipids under Two Types of Heat Stress. Plants (Basel). 2020; 9(6). PMC: 7356150. DOI: 10.3390/plants9060693. View

14.

Porcher A, Guerin V, Montrichard F, Lebrec A, Lothier J, Vian A . Ascorbate glutathione-dependent H2O2 scavenging is an important process in axillary bud outgrowth in rosebush. Ann Bot. 2020; 126(6):1049-1062. PMC: 7596375. DOI: 10.1093/aob/mcaa130. View

15.

Yuan C, Cai J . Time-series expression profile analysis of fracture healing in young and old mice. Mol Med Rep. 2017; 16(4):4529-4536. PMC: 5647013. DOI: 10.3892/mmr.2017.7198. View

16.

Hu Y, Han Z, Sun Y, Wang S, Wang T, Wang Y . ERF4 affects fruit firmness through TPL4 by reducing ethylene production. Plant J. 2020; 103(3):937-950. DOI: 10.1111/tpj.14884. View

17.

Wang K, Durrett T, Benning C . Functional diversity of glycerolipid acylhydrolases in plant metabolism and physiology. Prog Lipid Res. 2019; 75:100987. DOI: 10.1016/j.plipres.2019.100987. View

18.

Meng Q, Gao J, Zhu H, He H, Lu Z, Hong M . The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence. Biochem Biophys Res Commun. 2018; 505(4):1112-1120. DOI: 10.1016/j.bbrc.2018.10.065. View

19.

Yang C, Ding M, Shao G, Jia S, Yin X, Cui Y . Kcnk3, Ggta1, and Gpr84 are involved in hyperbaric oxygenation preconditioning protection on cerebral ischemia-reperfusion injury. Exp Brain Res. 2021; 239(12):3601-3613. PMC: 8599220. DOI: 10.1007/s00221-021-06220-7. View

20.

Dahn M, Dean C, Jo D, Coyle K, Marcato P . Human-specific GAPDH qRT-PCR is an accurate and sensitive method of xenograft metastasis quantification. Mol Ther Methods Clin Dev. 2021; 20:398-408. PMC: 7848707. DOI: 10.1016/j.omtm.2020.12.010. View