Ribosomal DNA Replication Time Coordinates Completion of Genome Replication and Anaphase in Yeast

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2023 Feb 26

PMID 36842087

Authors

Elizabeth X Kwan

Gina M Alvino

Kelsey L Lynch

Paula F Levan

Haley M Amemiya

Xiaobin S Wang

Sarah A Johnson

Joseph C Sanchez

Madison A Miller

Mackenzie Croy

Seung-Been Lee

Maria Naushab

Antonio Bedalov

Josh T Cuperus

Bonita J Brewer

Christine Queitsch

M K Raghuraman

Affiliations

Soon will be listed here.

Abstract

Timely completion of genome replication is a prerequisite for mitosis, genome integrity, and cell survival. A challenge to this timely completion comes from the need to replicate the hundreds of untranscribed copies of rDNA that organisms maintain in addition to the copies required for ribosome biogenesis. Replication of these rDNA arrays is relegated to late S phase despite their large size, repetitive nature, and essentiality. Here, we show that, in Saccharomyces cerevisiae, reducing the number of rDNA repeats leads to early rDNA replication, which results in delaying replication elsewhere in the genome. Moreover, cells with early-replicating rDNA arrays and delayed genome-wide replication aberrantly release the mitotic phosphatase Cdc14 from the nucleolus and enter anaphase prematurely. We propose that rDNA copy number determines the replication time of the rDNA locus and that the release of Cdc14 upon completion of rDNA replication is a signal for cell cycle progression.

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