in Sudan: Multidrug Resistance, Polyclonal Dissemination, and Virulence

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2023 Feb 25

PMID 36830144

Authors

Einas A Osman

Maho Yokoyama

Hisham N Altayb

Daire Cantillon

Julia Wille

Harald Seifert

Paul G Higgins

Leena Al-Hassan

Affiliations

Soon will be listed here.

Abstract

The emergence and global expansion of hyper-virulent and multidrug resistant (MDR) is an increasing healthcare threat worldwide. The epidemiology of MDR is under-characterized in many parts of the world, particularly Africa. In this study, isolates from hospitals in Khartoum, Sudan, have been whole-genome sequenced to investigate their molecular epidemiology, virulence, and resistome profiles. Eighty-six were recovered from patients in five hospitals in Khartoum between 2016 and 2020. Antimicrobial susceptibility was performed by disk-diffusion and broth microdilution. All isolates underwent whole genome sequencing using Illumina MiSeq; cgMLST was determined using Ridom SeqSphere+, and 7-loci MLST virulence genes and resistomes were identified. MDR was observed at 80%, with 35 isolates (41%) confirmed carbapenem-resistant. Thirty-seven sequence types were identified, and 14 transmission clusters (TC). Five of these TCs involved more than one hospital. was the most common virulence gene detected, in addition to some isolates harbouring and . There is a diverse population of in Khartoum hospitals, harbouring multiple resistance genes, including genes coding for ESBLs, carbapenemases, and aminoglycoside-modifying enzymes, across multiple ST's. The majority of isolates were singletons and transmissions were rare.

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