Comparing Conventional, Biochemical and Genotypic Methods for Accurate Identification of in Sudan

Overview

Journal Access Microbiol

Specialty Microbiology

Date 2020 Sep 25

PMID 32974573

Citations 11

Authors

Einas A Osman

Nagwa El-Amin

Emad A E Adrees

Leena Al-Hassan

Maowia Mukhtar

Affiliations

Soon will be listed here.

Abstract

is recognized as one of the most important healthcare-associated pathogens worldwide due to its tendency to develop antibiotic resistance and cause fatal outcomes. Bacterial identification methods such as culture and biochemical tests are routinely used with limited accuracy in many low- and middle-income countries, including Sudan. The aim of this study was to test the accuracy of identification of in Khartoum, Sudan. Two hundred and fifty isolates were collected and identified using conventional phenotypic methods, biochemically using API 20E and genotypically by amplification of 16S-23S rDNA and sequencing of , and . Only 139 (55.6 %) of the isolates were confirmed as genotypically by PCR and 44.4 % were identified as non- . The results demonstrate that the identification panels used by the hospitals were inaccurately identifying and led to overestimation of the prevalence of this organism. The current identification methods used in Khartoum hospitals are highly inaccurate, and therefore we recommend the use of a comprehensive biochemical panel or molecular methods, when possible, for accurate identification of .

Citing Articles

Genetic Insights on Meropenem Resistance Concerning Clinical Isolates.

Elkady F, Badr B, Alfeky A, Abdulrahman M, Hashem A, Al-Askar A Life (Basel). 2024; 14(11).

PMID: 39598206 PMC: 11595234. DOI: 10.3390/life14111408.

HPLC-PDA Analysis of Polyacetylene Glucosides from and Their Antibacterial and Antibiofilm Properties against .

Aljarba T, Bar F, Sherif A, Elekhnawy E, Magdy G, Samra R Pharmaceuticals (Basel). 2024; 17(9).

PMID: 39338376 PMC: 11434657. DOI: 10.3390/ph17091214.

Proteome analysis, genetic characterization, and antibiotic resistance patterns of Klebsiella pneumoniae clinical isolates.

Marzouk E, Abalkhail A, ALqahtani J, Alsowat K, Alanazi M, Alzaben F AMB Express. 2024; 14(1):54.

PMID: 38722429 PMC: 11082098. DOI: 10.1186/s13568-024-01710-7.

Antibiotic Susceptibility, Biofilm-Forming Ability, and Prevalence of Extended-Spectrum Beta-Lactamase (ESBL)- and Biofilm-Associated Genes Among Klebsiella pneumoniae Isolates from Hospitalized Patients in Northwest of Iran.

Mirzaei B, Ebrahimi A, Keshavarzi S, Hydarzadeh S, Badmasti F, Dadar M Curr Microbiol. 2023; 80(5):175.

PMID: 37029837 DOI: 10.1007/s00284-023-03247-7.

in Sudan: Multidrug Resistance, Polyclonal Dissemination, and Virulence.

Osman E, Yokoyama M, Altayb H, Cantillon D, Wille J, Seifert H Antibiotics (Basel). 2023; 12(2).

PMID: 36830144 PMC: 9952582. DOI: 10.3390/antibiotics12020233.

References

Nordmann P, Cuzon G, Naas T . The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infect Dis. 2009; 9(4):228-36. DOI: 10.1016/S1473-3099(09)70054-4. View

Podschun R, Ullmann U . Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev. 1998; 11(4):589-603. PMC: 88898. DOI: 10.1128/CMR.11.4.589. View

Claus H . Optimal selection of biochemical tests to identify microbial species. Zentralbl Bakteriol. 1993; 278(4):522-8. DOI: 10.1016/s0934-8840(11)80823-6. View

Diancourt L, Passet V, Verhoef J, Grimont P, Brisse S . Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. J Clin Microbiol. 2005; 43(8):4178-82. PMC: 1233940. DOI: 10.1128/JCM.43.8.4178-4182.2005. View

Ahmed S, Alp E, Ulu-Kilic A, Doganay M . Establishing molecular microbiology facilities in developing countries. J Infect Public Health. 2015; 8(6):513-25. DOI: 10.1016/j.jiph.2015.04.029. View

Liu Y, Liu C, Zheng W, Zhang X, Yu J, Gao Q . PCR detection of Klebsiella pneumoniae in infant formula based on 16S-23S internal transcribed spacer. Int J Food Microbiol. 2008; 125(3):230-5. DOI: 10.1016/j.ijfoodmicro.2008.03.005. View

Ng L, Tan T, Yeow S . A cost-effective method for the presumptive identification of Enterobacteriaceae for diagnostic microbiology laboratories. Pathology. 2010; 42(3):280-3. DOI: 10.3109/00313021003631338. View

Alves M, Dias R, de Castro A, Riley L, Moreira B . Identification of clinical isolates of indole-positive and indole-negative Klebsiella spp. J Clin Microbiol. 2006; 44(10):3640-6. PMC: 1594763. DOI: 10.1128/JCM.00940-06. View

Hansen D, Aucken H, Abiola T, Podschun R . Recommended test panel for differentiation of Klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical tests. J Clin Microbiol. 2004; 42(8):3665-9. PMC: 497635. DOI: 10.1128/JCM.42.8.3665-3669.2004. View

10.

Hamdan H, Kubbara E, Adam A, Hassan O, Suliman S, Adam I . Urinary tract infections and antimicrobial sensitivity among diabetic patients at Khartoum, Sudan. Ann Clin Microbiol Antimicrob. 2015; 14:26. PMC: 4406170. DOI: 10.1186/s12941-015-0082-4. View

11.

Wang M, Cao B, Yu Q, Liu L, Gao Q, Wang L . Analysis of the 16S-23S rRNA gene internal transcribed spacer region in Klebsiella species. J Clin Microbiol. 2008; 46(11):3555-63. PMC: 2576583. DOI: 10.1128/JCM.00927-08. View

12.

Lopes A, Rodrigues J, Clementino M, Miranda C, Nascimento A, Antonio de Morais Junior M . Application of PCR ribotyping and tDNA-PCR for Klebsiella pneumoniae identification. Mem Inst Oswaldo Cruz. 2007; 102(7):827-32. DOI: 10.1590/s0074-02762007005000113. View

13.

Ko W, Paterson D, Sagnimeni A, Hansen D, von Gottberg A, Mohapatra S . Community-acquired Klebsiella pneumoniae bacteremia: global differences in clinical patterns. Emerg Infect Dis. 2002; 8(2):160-6. PMC: 2732457. DOI: 10.3201/eid0802.010025. View