A Simple and Cost-effective Method for Studying Anoxia Tolerance in Plants

Overview

Journal Appl Plant Sci

Date 2023 Feb 23

PMID 36818780

Authors

Orla L Sherwood

Rebecca Carroll

Stephen Burke

Paul F McCabe

Joanna Kacprzyk

Affiliations

Soon will be listed here.

Abstract

Premise: We developed a novel, cost-effective protocol that facilitates testing anoxia tolerance in plants without access to specialized equipment.

Methods And Results: and barley () seedlings were treated in airtight 2-L Kilner jars. An anoxic atmosphere was generated using Oxoid AnaeroGen 2.5-L sachets placed on in-house, custom-built wire stands. The performed experiments confirmed a higher sensitivity to low oxygen stress previously observed in mutants and the positive effect of exogenous sucrose on anoxia tolerance reported by previous studies in . Barley seedlings displayed typical responses to anoxia treatment, including shoot growth cessation and the induction of marker genes for anaerobic metabolism and ethylene biosynthesis in root tissue.

Conclusions: The results validate the novel method as an inexpensive, simple alternative for testing anoxia tolerance in plants, where access to an anaerobic workstation is not possible. The novel protocol requires minimum investment and is easily adaptable.

Citing Articles

A simple and cost-effective method for studying anoxia tolerance in plants.

Sherwood O, Carroll R, Burke S, McCabe P, Kacprzyk J Appl Plant Sci. 2023; 11(1):e11509.

PMID: 36818780 PMC: 9934590. DOI: 10.1002/aps3.11509.

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