Novel Prognostic Biomarkers for Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma (CESC) Patients Via Analysis of Competing Endogenous RNA (ceRNA) Network

Overview

Journal Dis Markers

Publisher Wiley

Specialty Biochemistry

Date 2023 Feb 23

PMID 36817087

Authors

Yanru Dong

Weibo Wen

Tiezheng Yuan

Lan Liu

Xiangdan Li

Affiliations

Soon will be listed here.

Abstract

Background: Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) is a common malignant gynecological cancer. The ceRNA networks play important roles in many tumors, while RILPL2-related ceRNA network has been seldom studied in CESC.

Methods: All CESC data was obtained from TCGA database. Differentially expressed RNAs and predicted target RNAs were cross analyzed to construct ceRNA network. RNA and clinicopathological characteristics' influence on overall survival (OS) were determined by univariate and multivariate Cox regression analyses. Lasso regression was used to construct the prediction model. Coexpression analysis was performed to explore the association of gene expression with CESC. This was followed by an experimental validation based on these results.

Results: Between high and low RILPL2 expression CESC patients, totally 1227 DEmRNAs, 39 DEmiRNAs, and 1544 DElncRNAs were identified. After multiple cross analyses, 1 miRNA hsa-miR-1293, 20 mRNAs, and 43 lncRNAs were maintained to construct ceRNA network. CADM3-AS1, LINC00092, and ZNF667-AS1 in ceRNA network were significantly associated with the OS of CESC patients, and patients with low expression of these lncRNAs had worse prognosis. Significant lower expressions of these lncRNAs were also observed in CESC cell line compared with normal cell line.

Conclusion: Low expressions of CADM3-AS1, LINC00092, and ZNF667-AS1 in ceRNA network were probably promising poor prognostic biomarkers for CESC patients. The genes show a prospective research area for CESC-targeted treatment in the future.

Citing Articles

Unraveling the role of the circadian clock genes in cervical squamous cell carcinoma and endocervical adenocarcinoma: A prognostic indicator for prognostic, immunotherapy response, and chemotherapy sensitivity.

Xu J, Huang X, Gou S, Luo H, Zeng S, Zhang Q J Cancer. 2024; 15(9):2788-2804.

PMID: 38577592 PMC: 10988312. DOI: 10.7150/jca.94063.

Retracted: Novel Prognostic Biomarkers for Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma (CESC) Patients via Analysis of Competing Endogenous RNA (ceRNA) Network.

Markers D Dis Markers. 2023; 2023:9841329.

PMID: 37538089 PMC: 10396544. DOI: 10.1155/2023/9841329.

References

Wang X, Wang X, Huang G, Chen Y . Lower expression of LINC00092 in lung adenocarcinoma might mean poorer prognosis: A study based on data mining and bioinformatics. Medicine (Baltimore). 2020; 99(44):e23012. PMC: 7598869. DOI: 10.1097/MD.0000000000023012. View

Joshi P, Esko T, Mattsson H, Eklund N, Gandin I, Nutile T . Directional dominance on stature and cognition in diverse human populations. Nature. 2015; 523(7561):459-462. PMC: 4516141. DOI: 10.1038/nature14618. View

Yoon J, Abdelmohsen K, Gorospe M . Functional interactions among microRNAs and long noncoding RNAs. Semin Cell Dev Biol. 2014; 34:9-14. PMC: 4163095. DOI: 10.1016/j.semcdb.2014.05.015. View

Zhou R, Zhang E, Sun Q, Ye Z, Liu J, Zhou D . Integrated analysis of lncRNA-miRNA-mRNA ceRNA network in squamous cell carcinoma of tongue. BMC Cancer. 2019; 19(1):779. PMC: 6686570. DOI: 10.1186/s12885-019-5983-8. View

Farazi T, Hoell J, Morozov P, Tuschl T . MicroRNAs in human cancer. Adv Exp Med Biol. 2013; 774:1-20. PMC: 3704221. DOI: 10.1007/978-94-007-5590-1_1. View

Wang T, Zhang X, Hua K . A ceRNA network of BBOX1-AS1-hsa-miR-125b-5p/hsa-miR-125a-5p-CDKN2A shows prognostic value in cervical cancer. Taiwan J Obstet Gynecol. 2021; 60(2):253-261. DOI: 10.1016/j.tjog.2020.12.006. View

Salmena L, Poliseno L, Tay Y, Kats L, Pandolfi P . A ceRNA hypothesis: the Rosetta Stone of a hidden RNA language?. Cell. 2011; 146(3):353-8. PMC: 3235919. DOI: 10.1016/j.cell.2011.07.014. View

Kulkarni P, Rani H, Vimalambike M, Ravishankar S . Opportunistic screening for cervical cancer in a tertiary hospital in Karnataka, India. Asian Pac J Cancer Prev. 2013; 14(9):5101-5. DOI: 10.7314/apjcp.2013.14.9.5101. View

Small Jr W, Bacon M, Bajaj A, Chuang L, Fisher B, Harkenrider M . Cervical cancer: A global health crisis. Cancer. 2017; 123(13):2404-2412. DOI: 10.1002/cncr.30667. View

10.

Sun X, Chen D, Jin Z, Chen T, Lin A, Jin H . Genome-wide methylation and expression profiling identify methylation-associated genes in colorectal cancer. Epigenomics. 2019; 12(1):19-36. DOI: 10.2217/epi-2019-0133. View

11.

Shi X, Li X, Zhang H, He R, Zhao Y, Zhou M . A Five-microRNA Signature for Survival Prognosis in Pancreatic Adenocarcinoma based on TCGA Data. Sci Rep. 2018; 8(1):7638. PMC: 5955976. DOI: 10.1038/s41598-018-22493-5. View

12.

Ding H, Xiong X, Fan G, Yi Y, Chen Y, Wang J . The New Biomarker for Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma (CESC) Based on Public Database Mining. Biomed Res Int. 2020; 2020:5478574. PMC: 7174939. DOI: 10.1155/2020/5478574. View

13.

Li Y, Yang Z, Wang Y, Wang Y . Long noncoding RNA ZNF667-AS1 reduces tumor invasion and metastasis in cervical cancer by counteracting microRNA-93-3p-dependent PEG3 downregulation. Mol Oncol. 2019; 13(11):2375-2392. PMC: 6822248. DOI: 10.1002/1878-0261.12565. View

14.

Agarwal V, Bell G, Nam J, Bartel D . Predicting effective microRNA target sites in mammalian mRNAs. Elife. 2015; 4. PMC: 4532895. DOI: 10.7554/eLife.05005. View

15.

Ding H, Zhang L, Zhang C, Song J, Jiang Y . Screening of Significant Biomarkers Related to Prognosis of Cervical Cancer and Functional Study Based on lncRNA-associated ceRNA Regulatory Network. Comb Chem High Throughput Screen. 2020; 24(3):472-482. DOI: 10.2174/1386207323999200729113028. View

16.

Paavonen J, Naud P, Salmeron J, Wheeler C, Chow S, Apter D . Efficacy of human papillomavirus (HPV)-16/18 AS04-adjuvanted vaccine against cervical infection and precancer caused by oncogenic HPV types (PATRICIA): final analysis of a double-blind, randomised study in young women. Lancet. 2009; 374(9686):301-14. DOI: 10.1016/S0140-6736(09)61248-4. View

17.

Fan Y, Sheng W, Meng Y, Cao Y, Li R . LncRNA PTENP1 inhibits cervical cancer progression by suppressing miR-106b. Artif Cells Nanomed Biotechnol. 2020; 48(1):393-407. DOI: 10.1080/21691401.2019.1709852. View

18.

Tang Z, Li C, Kang B, Gao G, Li C, Zhang Z . GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 2017; 45(W1):W98-W102. PMC: 5570223. DOI: 10.1093/nar/gkx247. View

19.

Heintz A, Odicino F, Maisonneuve P, Quinn M, Benedet J, Creasman W . Carcinoma of the Fallopian Tube. Int J Gynaecol Obstet. 2018; 95 Suppl 1:S145-S160. DOI: 10.1016/S0020-7292(06)60032-5. View

20.

Luan X, Wang Y . LncRNA XLOC_006390 facilitates cervical cancer tumorigenesis and metastasis as a ceRNA against miR-331-3p and miR-338-3p. J Gynecol Oncol. 2018; 29(6):e95. PMC: 6189437. DOI: 10.3802/jgo.2018.29.e95. View