Analysis of Mitochondrial DNA Replication by Two-Dimensional Agarose Gel Electrophoresis

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Feb 22

PMID 36807797

Authors

Steffi Goffart

Jaakko Pohjoismaki

Affiliations

Soon will be listed here.

Abstract

Two-dimensional neutral/neutral agarose gel electrophoresis (2D-AGE) has been employed for nearly two decades in the analysis of replication and maintenance processes of animal mitochondrial DNA, but the method's potential has not been fully exploited. Here, we describe the various steps involved in this technique, from DNA isolation, to two-dimensional neutral/neutral agarose gel electrophoresis (2D-AGE), Southern hybridization and interpretation. We also provide examples of the applicability of 2D-AGE to investigate the different features of mtDNA maintenance and regulation.

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