Combined Use of Magnetic Microbeads for Endothelial Cell Isolation and Enhanced Cell Engraftment in Myocardial Repair

Overview

Journal Theranostics

Date 2023 Feb 16

PMID 36793861

Authors

Christian Biederbick

Jan C Heinemann

Sarah Rieck

Florian Winkler

Annika Ottersbach

Miriam Schiffer

Georg D Duerr

Dietmar Eberbeck

Michael Hesse

Wilhelm Roll

Daniela Wenzel

Affiliations

Soon will be listed here.

Abstract

The regenerative potential of the heart after injury is limited. Therefore, cell replacement strategies have been developed. However, the engraftment of transplanted cells in the myocardium is very inefficient. In addition, the use of heterogeneous cell populations precludes the reproducibility of the outcome. To address both issues, in this proof of principle study, we applied magnetic microbeads for combined isolation of eGFP embryonic cardiac endothelial cells (CECs) by antigen-specific magnet-associated cell sorting (MACS) and improved engraftment of these cells in myocardial infarction by magnetic fields. MACS provided CECs of high purity decorated with magnetic microbeads. experiments revealed that the angiogenic potential of microbead-labeled CECs was preserved and the magnetic moment of the cells was strong enough for site-specific positioning by a magnetic field. After myocardial infarction in mice, intramyocardial CEC injection in the presence of a magnet resulted in a strong improvement of cell engraftment and eGFP vascular network formation in the hearts. Hemodynamic and morphometric analysis demonstrated augmented heart function and reduced infarct size only when a magnetic field was applied. Thus, the combined use of magnetic microbeads for cell isolation and enhanced cell engraftment in the presence of a magnetic field is a powerful approach to improve cell transplantation strategies in the heart.

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