Engineering of an Electrically Charged Hydrogel Implanted into a Traumatic Brain Injury Model for Stepwise Neuronal Tissue Reconstruction

Overview

Journal Sci Rep

Specialty Science

Date 2023 Feb 14

PMID 36788295

Authors

Satoshi Tanikawa

Yuki Ebisu

Tomas Sedlacik

Shingo Semba

Takayuki Nonoyama

Takayuki Kurokawa

Akira Hirota

Taiga Takahashi

Kazushi Yamaguchi

Masamichi Imajo

Hinako Kato

Takuya Nishimura

Zen-Ichi Tanei

Masumi Tsuda

Tomomi Nemoto

Jian Ping Gong

Shinya Tanaka

Affiliations

Soon will be listed here.

Abstract

Neural regeneration is extremely difficult to achieve. In traumatic brain injuries, the loss of brain parenchyma volume hinders neural regeneration. In this study, neuronal tissue engineering was performed by using electrically charged hydrogels composed of cationic and anionic monomers in a 1:1 ratio (C1A1 hydrogel), which served as an effective scaffold for the attachment of neural stem cells (NSCs). In the 3D environment of porous C1A1 hydrogels engineered by the cryogelation technique, NSCs differentiated into neuroglial cells. The C1A1 porous hydrogel was implanted into brain defects in a mouse traumatic damage model. The VEGF-immersed C1A1 porous hydrogel promoted host-derived vascular network formation together with the infiltration of macrophages/microglia and astrocytes into the gel. Furthermore, the stepwise transplantation of GFP-labeled NSCs supported differentiation towards glial and neuronal cells. Therefore, this two-step method for neural regeneration may become a new approach for therapeutic brain tissue reconstruction after brain damage in the future.

Citing Articles

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