Effect of Zinc Oxide Nanoparticles Synthesized from Leaf Extract on Growth and Antioxidant Properties of Mustard ()

Overview

Journal Front Plant Sci

Date 2023 Feb 9

PMID 36755696

Authors

Addisie Geremew

Laura Carson

Selamawit Woldesenbet

Huichen Wang

Sheena Reeves

Nigel Brooks Jr

Premkumar Saganti

Aruna Weerasooriya

Elisha Peace

Affiliations

Soon will be listed here.

Abstract

Background: The sustainability of crop production is impacted by climate change and land degradation, and the advanced application of nanotechnology is of paramount importance to overcome this challenge. The development of nanomaterials based on essential nutrients like zinc could serve as a basis for nanofertilizers and nanocomposite synthesis for broader agricultural applications and quality human nutrition. Therefore, this study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using pecan (Carya illinoinensis) leaf extract and investigate their effect on the growth, physiology, nutrient content, and antioxidant properties of mustard (Brassica juncea).

Methods: The ZnO NPs were characterized by UV-Vis spectrophotometry, Dynamic Light Scattering (DLS), X-ray diffractometer (XRD), Scanning Electron Microscopy (SEM), and Fourier Transform Infra-Red Spectroscopy (FTIR). Mustard plants were subjected to different concentrations of ZnONPs (0, 20, 40, 60, 80, 100 and 200 mg L-1) during the vegetative growth stage.

Results: The UV-Vis spectra of ZnO NPs revealed the absorption maxima at 362 nm and FTIR identified numerous functional groups that are responsible for capping and stabilizing ZnO NPs. DLS analysis presented monodispersed ZnO NPs of 84.5 nm size and highly negative zeta potential (-22.4 mV). Overall, the application of ZnO NPs enhanced the growth, chlorophyll content (by 53 %), relative water content (by 46 %), shoot biomass, membrane stability (by 54 %) and net photosynthesis significantly in a dose-dependent manner. In addition, the supplement of the ZnO NPs augmented K, Fe, Zn and flavonoid contents as well as overcome the effect of reactive oxygen species by increasing antioxidant capacity in mustard leaves up to 97 %.

Conclusions: In conclusion, ZnO NPs can be potentially used as a plant growth stimulant and as a novel soil amendment for enhancing crop yields. Besides, the biofortification of B. juncea plants with ZnO NPs helps to improve the nutritional quality of the crop and perhaps potentiates its pharmaceutical effects.

Citing Articles

Nanoparticles in Plant Cryopreservation: Effects on Genetic Stability, Metabolic Profiles, and Structural Integrity in Bleeding Heart (Papaveraceae) Cultivars.

Kulus D, Tymoszuk A, Kulpinska A, Debska B, Michalska A, Nowakowska J Nanotechnol Sci Appl. 2025; 18:35-56.

PMID: 39989598 PMC: 11844321. DOI: 10.2147/NSA.S485428.

Foliar spraying with zinc oxide nanoparticles enhances the anti-osteoporotic efficacy of the fruit extracts of L. by stimulating silybin production.

Fahad Almulhim B, Sherif F, Younis N, Safwat Y, Khattab S Front Plant Sci. 2025; 15():1421485.

PMID: 39840357 PMC: 11747799. DOI: 10.3389/fpls.2024.1421485.

Green Synthesis and Characterization of Zinc Oxide Nanoparticles Biosynthesized from Butea monosperma Flowers and Glycyrrhiza glabra Roots and their Antioxidant and Antibacterial Properties.

Alam K, Din I, Tariq S, Hayat K, Khan F, Khan M Appl Biochem Biotechnol. 2024; .

PMID: 39601975 DOI: 10.1007/s12010-024-05102-2.

Zn-Al and Mg-Al layered double hydroxide nanoparticles improved primary and secondary metabolism of geranium plants.

Hashem S, AbdElgawad H, Mohamed F, Hegab M, AlGarawi A, Okla M RSC Adv. 2024; 14(39):28376-28389.

PMID: 39239289 PMC: 11375793. DOI: 10.1039/d4ra04280h.

An Outlook on Platinum-Based Active Ingredients for Dermatologic and Skincare Applications.

Li S, Liu Y, Wu Y, Ren L, Lu Y, Yamaguchi S Nanomaterials (Basel). 2024; 14(15).

PMID: 39120408 PMC: 11314049. DOI: 10.3390/nano14151303.

References

Rajput V, Minkina T, Kumari A, Harish , Singh V, Verma K . Coping with the Challenges of Abiotic Stress in Plants: New Dimensions in the Field Application of Nanoparticles. Plants (Basel). 2021; 10(6). PMC: 8232821. DOI: 10.3390/plants10061221. View

Raliya R, Chandra Tarafdar J, Biswas P . Enhancing the Mobilization of Native Phosphorus in the Mung Bean Rhizosphere Using ZnO Nanoparticles Synthesized by Soil Fungi. J Agric Food Chem. 2016; 64(16):3111-8. DOI: 10.1021/acs.jafc.5b05224. View

Majdoub Y, Alibrando F, Cacciola F, Arena K, Pagnotta E, Matteo R . Chemical Characterization of Three Accessions of L. Extracts from Different Plant Tissues. Molecules. 2020; 25(22). PMC: 7699538. DOI: 10.3390/molecules25225421. View

Gill S, Tuteja N . Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010; 48(12):909-30. DOI: 10.1016/j.plaphy.2010.08.016. View

Narayana A, Bhat S, Fathima A, Lokesh S, Surya S, Yelamaggad C . Green and low-cost synthesis of zinc oxide nanoparticles and their application in transistor-based carbon monoxide sensing. RSC Adv. 2022; 10(23):13532-13542. PMC: 9051533. DOI: 10.1039/d0ra00478b. View

Schwab F, Zhai G, Kern M, Turner A, Schnoor J, Wiesner M . Barriers, pathways and processes for uptake, translocation and accumulation of nanomaterials in plants--Critical review. Nanotoxicology. 2015; 10(3):257-78. DOI: 10.3109/17435390.2015.1048326. View

Mukherjee A, Sun Y, Morelius E, Tamez C, Bandyopadhyay S, Niu G . Differential Toxicity of Bare and Hybrid ZnO Nanoparticles in Green Pea (Pisum sativum L.): A Life Cycle Study. Front Plant Sci. 2016; 6:1242. PMC: 4710101. DOI: 10.3389/fpls.2015.01242. View

Antoniou C, Savvides A, Christou A, Fotopoulos V . Unravelling chemical priming machinery in plants: the role of reactive oxygen-nitrogen-sulfur species in abiotic stress tolerance enhancement. Curr Opin Plant Biol. 2016; 33:101-107. DOI: 10.1016/j.pbi.2016.06.020. View

Adil M, Khan T, Aasim M, Khan A, Ashraf M . Evaluation of the antibacterial potential of silver nanoparticles synthesized through the interaction of antibiotic and aqueous callus extract of Fagonia indica. AMB Express. 2019; 9(1):75. PMC: 6536562. DOI: 10.1186/s13568-019-0797-2. View

10.

Ulhassan Z, Gill R, Huang H, Ali S, Mwamba T, Ali B . Selenium mitigates the chromium toxicity in Brassicca napus L. by ameliorating nutrients uptake, amino acids metabolism and antioxidant defense system. Plant Physiol Biochem. 2019; 145:142-152. DOI: 10.1016/j.plaphy.2019.10.035. View

11.

Ilboudo O, Tapsoba I, Bonzi-Coulibaly Y, Gerbaux P . Targeting structural motifs of flavonoid diglycosides using collision-induced dissociation experiments on flavonoid/Pb2+ complexes. Eur J Mass Spectrom (Chichester). 2012; 18(5):465-73. DOI: 10.1255/ejms.1199. View

12.

Yu J, Huang L, Hu W, Zhou Y, Mao W, Ye S . A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus. J Exp Bot. 2004; 55(399):1135-43. DOI: 10.1093/jxb/erh124. View

13.

Hasanuzzaman M, Bhuyan M, Zulfiqar F, Raza A, Mohsin S, Mahmud J . Reactive Oxygen Species and Antioxidant Defense in Plants under Abiotic Stress: Revisiting the Crucial Role of a Universal Defense Regulator. Antioxidants (Basel). 2020; 9(8). PMC: 7465626. DOI: 10.3390/antiox9080681. View

14.

Chen J, Dou R, Yang Z, You T, Gao X, Wang L . Phytotoxicity and bioaccumulation of zinc oxide nanoparticles in rice (Oryza sativa L.). Plant Physiol Biochem. 2018; 130:604-612. DOI: 10.1016/j.plaphy.2018.08.019. View

15.

El-Aziz M, Morsi S, Salama D, Abdel-Aziz M, Abd Elwahed M, Shaaban E . Preparation and characterization of chitosan/polyacrylic acid/copper nanocomposites and their impact on onion production. Int J Biol Macromol. 2018; 123:856-865. DOI: 10.1016/j.ijbiomac.2018.11.155. View

16.

Hussain F, Hadi F, Rongliang Q . Effects of zinc oxide nanoparticles on antioxidants, chlorophyll contents, and proline in Persicaria hydropiper L. and its potential for Pb phytoremediation. Environ Sci Pollut Res Int. 2021; 28(26):34697-34713. DOI: 10.1007/s11356-021-13132-0. View

17.

Lukacova Z, Bokor B, Vavrova S, Soltys K, Vaculik M . Divergence of reactions to arsenic (As) toxicity in tobacco (Nicotiana benthamiana) plants: A lesson from peroxidase involvement. J Hazard Mater. 2021; 417:126049. DOI: 10.1016/j.jhazmat.2021.126049. View

18.

Rastogi A, Zivcak M, Sytar O, Kalaji H, He X, Mbarki S . Impact of Metal and Metal Oxide Nanoparticles on Plant: A Critical Review. Front Chem. 2017; 5:78. PMC: 5643474. DOI: 10.3389/fchem.2017.00078. View

19.

Govorov A, Carmeli I . Hybrid structures composed of photosynthetic system and metal nanoparticles: plasmon enhancement effect. Nano Lett. 2007; 7(3):620-5. DOI: 10.1021/nl062528t. View

20.

Chai H, Yao J, Sun J, Zhang C, Liu W, Zhu M . The effect of metal oxide nanoparticles on functional bacteria and metabolic profiles in agricultural soil. Bull Environ Contam Toxicol. 2015; 94(4):490-5. DOI: 10.1007/s00128-015-1485-9. View