Regeneration of Humeral Head Using a 3D Bioprinted Anisotropic Scaffold with Dual Modulation of Endochondral Ossification

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2023 Feb 9

PMID 36755334

Authors

Tao Li

Zhengjiang Ma

Yuxin Zhang

Zezheng Yang

Wentao Li

Dezhi Lu

Yihao Liu

Lei Qiang

Tianchang Wang

Ya Ren

Wenhao Wang

Hongtao He

Xiaojun Zhou

Yuanqing Mao

Junfeng Zhu

Jinwu Wang

Xiaodong Chen

Kerong Dai

Affiliations

Soon will be listed here.

Abstract

Tissue engineering is theoretically thought to be a promising method for the reconstruction of biological joints, and thus, offers a potential treatment alternative for advanced osteoarthritis. However, to date, no significant progress is made in the regeneration of large biological joints. In the current study, a biomimetic scaffold for rabbit humeral head regeneration consisting of heterogeneous porous architecture, various bioinks, and different hard supporting materials in the cartilage and bone regions is designed and fabricated in one step using 3D bioprinting technology. Furthermore, orchestrated dynamic mechanical stimulus combined with different biochemical cues (parathyroid hormone [PTH] and chemical component hydroxyapatite [HA] in the outer and inner region, respectively) are used for dual regulation of endochondral ossification. Specifically, dynamic mechanical stimulus combined with growth factor PTH in the outer region inhibits endochondral ossification and results in cartilage regeneration, whereas dynamic mechanical stimulus combined with HA in the inner region promotes endochondral ossification and results in efficient subchondral bone regeneration. The strategy established in this study with the dual modulation of endochondral ossification for 3D bioprinted anisotropic scaffolds represents a versatile and scalable approach for repairing large joints.

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