Quantification of Cardiomyocyte Contraction In Vitro and Drug Screening by MyocytoBeats

Overview

Journal J Cardiovasc Transl Res

Publisher Springer

Specialty Cardiology & Vascular Diseases

Date 2023 Jan 30

PMID 36715820

Authors

Zhiyang Cheng

Yuxin Yang

Kai Jiang

Hongyi Nie

Xingbo Yang

Zizhuo Tu

Jiayi Liang

Yaozu Xiang

Affiliations

Soon will be listed here.

Abstract

Cardiomyocyte contractility is the crucial feature of heart function. Quantifying cardiomyocyte contraction in vitro is essential for disease phenotype characterization, mechanism illumination, and drug screening. Although many experimental methods have been employed to determine contraction dynamics in vitro, a time-saving and easy-to-use software is still needed to be developed. We presented a reliable tool, named MyocytoBeats, to measure cardiomyocyte contraction by processing recorded videos. Analysis results by MyocytoBeats of various experimental models have shown a significant linear relationship with another validated software. We also performed pharmacology screen in the platform, and astragaloside IV was identified to stabilize the frequency and amplitude of cardiomyocyte in the arrhythmia model. MyocytoBeats is a high-performance tool for generating cardiomyocyte contraction data of vitro study and shows a great potential in cardiac pharmacology study.

Citing Articles

MicroBundleCompute: Automated segmentation, tracking, and analysis of subdomain deformation in cardiac microbundles.

Kobeissi H, Jilberto J, Karakan M, Gao X, DePalma S, Das S PLoS One. 2024; 19(3):e0298863.

PMID: 38530829 PMC: 10965069. DOI: 10.1371/journal.pone.0298863.

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