Low-volume Label-free SARS-CoV-2 Detection with the Microcavity-based Optical Fiber Sensor

Overview

Journal Sci Rep

Specialty Science

Date 2023 Jan 27

PMID 36707671

Authors

Monika Janik

Tomasz Gabler

Marcin Koba

Miroslawa Panasiuk

Yanina Dashkevich

Tomasz Lega

Agnieszka Dabrowska

Antonina Naskalska

Sabina Zoledowska

Dawid Nidzworski

Krzysztof Pyrc

Beata Gromadzka

Mateusz Smietana

Affiliations

Soon will be listed here.

Abstract

Accurate and fast detection of viruses is crucial for controlling outbreaks of many diseases; therefore, to date, numerous sensing systems for their detection have been studied. On top of the performance of these sensing systems, the availability of biorecognition elements specific to especially the new etiological agents is an additional fundamental challenge. Therefore, besides high sensitivity and selectivity, such advantages as the size of the sensor and possibly low volume of analyzed samples are also important, especially at the stage of evaluating the receptor-target interactions in the case of new etiological agents when typically, only tiny amounts of the receptor are available for testing. This work introduces a real-time, highly miniaturized sensing solution based on microcavity in-line Mach-Zehnder interferometer (μIMZI) induced in optical fiber for SARS-CoV-2 virus-like particles detection. The assay is designed to detect conserved regions of the SARS-CoV-2 viral particles in a sample with a volume as small as hundreds of picoliters, reaching the detection limit at the single ng per mL level.

Citing Articles

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