Evaluation of the Osteogenic Effect of Apigenin on Human Mesenchymal Stem Cells by Inhibiting Inflammation Through Modulation of NF-κB/IκBα

Overview

Journal Res Pharm Sci

Specialty Chemistry

Date 2023 Jan 27

PMID 36704428

Authors

Azita Asadi

Farjam Goudarzi

Mustafa Ghanadian

Adel Mohammadalipour

Affiliations

Soon will be listed here.

Abstract

Background And Purpose: Apigenin has stimulatory effects on osteogenic differentiation of human mesenchymal stem cells (hMSCs) as well as anti-inflammatory properties. This study investigated the osteogenic differentiation of hMSCs in inflammatory conditions treated with apigenin focusing on nuclear factor kappa-light-chain-enhancer of activated B (NF-кB), nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) and nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3 (NLRP3) inflammatory pathways.

Experimental Approach: Along with osteogenic differentiation of the hMSCs, they became inflamed with lipopolysaccharide (LPS)/palmitic acid (PA) and treated with apigenin. Alizarin red staining, alkaline phosphatase (ALP) activity, and Runt-related transcription factor 2 (RUNX2) gene expression were used to determine the degree of differentiation. Also, gene expression of NLRP3 was performed along with protein expression of interleukin 1-beta (IL-1β), NF-кB, and IκBα.

Findings / Results: Apigenin was shown to be effective in neutralizing the inhibitory impact of LPS/PA on osteogenesis. Apigenin increased MSC osteogenic capacity by inhibiting NLRP3 expression and the activity of caspase-1. It was also associated with a considerable decrease in the protein expression of NF-κB and IκBα, as well as IL-1β, in these cells.

Conclusion And Implications: The effects of apigenin on osteogenesis under inflammatory conditions were cautiously observed.

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