Supramolecular Polyphenol-DNA Microparticles for In Vivo Adjuvant and Antigen Co-Delivery and Immune Stimulation

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2023 Jan 26

PMID 36700351

Authors

Yijiao Qu

Robert De Rose

Chan-Jin Kim

Jiajing Zhou

Zhixing Lin

Yi Ju

Sukhvir Kaur Bhangu

Christina Cortez-Jugo

Francesca Cavalieri

Frank Caruso

Affiliations

Soon will be listed here.

Abstract

DNA-based materials have attracted interest due to the tunable structure and encoded biological functionality of nucleic acids. A simple and general approach to synthesize DNA-based materials with fine control over morphology and bioactivity is important to expand their applications. Here, we report the synthesis of DNA-based particles via the supramolecular assembly of tannic acid (TA) and DNA. Uniform particles with different morphologies are obtained using a variety of DNA building blocks. The particles enable the co-delivery of cytosine-guanine adjuvant sequences and the antigen ovalbumin in model cells. Intramuscular injection of the particles in mice induces antigen-specific antibody production and T cell responses with no apparent toxicity. Protein expression in cells is shown using capsules assembled from TA and plasmid DNA. This work highlights the potential of TA as a universal material for directing the supramolecular assembly of DNA into gene and vaccine delivery platforms.

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