Ramadan Diurnal Intermittent Fasting is Associated with Significant Plasma Metabolomics Changes in Subjects with Overweight and Obesity: A Prospective Cohort Study

Overview

Journal Front Nutr

Specialty Nutritional Sciences

Date 2023 Jan 26

PMID 36698470

Authors

Mohamed Madkour

Alexander D Giddey

Nelson C Soares

Mohammad H Semreen

Yasser Bustanji

Falak Zeb

Rabih Halwani

MoezAlIslam E Faris

Affiliations

Soon will be listed here.

Abstract

Introduction: During the holy month of Ramadan, adult healthy Muslims are mandated to abstain from dawn to sunset, with free eating at night hours that may extend up to 12 h. The current work was designed to investigate the metabolomics changes incurred upon the observance of Ramadan diurnal intermittent fasting (RDIF).

Methods: Twenty-five metabolically healthy participants with overweight and obesity (7 females and 18 males, with a mean age of 39.48 ± 10.0 years) were recruited for the study and were followed before and at the end of RDIF month. Dietary, anthropometric, biochemical, and physical activity assessments were performed before and at the end of the fasting month. The metabolomic assay was performed using liquid chromatography-mass spectrometry for the two-time points.

Results And Discussion: Metabolomics assay revealed a significant reduction in a few metabolites. The analysis revealed that 27 metabolites differed significantly ( < 0.05) between pre-and post-RDIF. Among the differentially abundant metabolites, 23 showed a decrease with fasting, these included several amino acids such as aspartame, tryptophan, phenylalanine, histidine, and other metabolites including valeric acid, and cortisol. On the other hand, only four metabolites showed increased levels after RDIF including traumatic acid, 2-pyrrolidinone, PC[18:1(9Z)/18:1(9Z)], and . The MetaboAnalyst platform reported that the top enriched metabolic pathways included: (1) histidine metabolism; (2) folate biosynthesis (3) phenylalanine, tyrosine, and tryptophan biosynthesis; (4) aminoacyltRNA biosynthesis; (5) caffeine metabolism; (6) vitamin B metabolism; and several other pathways relating to lipid metabolisms such as arachidonic acid metabolism, glycerophospholipid metabolism, and linoleic acid metabolism. In conclusion, RDIF entails significant changes in various metabolic pathways that reflect different dietary and lifestyle behaviors practiced during the fasting month.

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References

Mindikoglu A, Abdulsada M, Jain A, Jalal P, Devaraj S, Wilhelm Z . Intermittent fasting from dawn to sunset for four consecutive weeks induces anticancer serum proteome response and improves metabolic syndrome. Sci Rep. 2020; 10(1):18341. PMC: 7592042. DOI: 10.1038/s41598-020-73767-w. View

Ooi T, Meramat A, Rajab N, Shahar S, Safinar Ismail I, Ahmad Azam A . Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study. Nutrients. 2020; 12(9). PMC: 7551340. DOI: 10.3390/nu12092644. View

Faris M, Hussein R, Al-Kurd R, Al-Fararjeh M, Bustanji Y, Khalil Mohammad M . Impact of ramadan intermittent fasting on oxidative stress measured by urinary 15-f(2t)-isoprostane. J Nutr Metab. 2012; 2012:802924. PMC: 3485525. DOI: 10.1155/2012/802924. View

Syme C, Czajkowski S, Shin J, Abrahamowicz M, Leonard G, Perron M . Glycerophosphocholine Metabolites and Cardiovascular Disease Risk Factors in Adolescents: A Cohort Study. Circulation. 2016; 134(21):1629-1636. DOI: 10.1161/CIRCULATIONAHA.116.022993. View

Siracusa R, Impellizzeri D, Cordaro M, Gugliandolo E, Peritore A, Di Paola R . Topical Application of Adelmidrol + -Traumatic Acid Enhances Skin Wound Healing in a Streptozotocin-Induced Diabetic Mouse Model. Front Pharmacol. 2018; 9:871. PMC: 6115498. DOI: 10.3389/fphar.2018.00871. View

Teruya T, Chaleckis R, Takada J, Yanagida M, Kondoh H . Diverse metabolic reactions activated during 58-hr fasting are revealed by non-targeted metabolomic analysis of human blood. Sci Rep. 2019; 9(1):854. PMC: 6351603. DOI: 10.1038/s41598-018-36674-9. View

Kaufman S . An evaluation of the possible neurotoxicity of metabolites of phenylalanine. J Pediatr. 1989; 114(5):895-900. DOI: 10.1016/s0022-3476(89)80161-1. View

Faris M, Jahrami H, Alhayki F, Alkhawaja N, Ali A, Aljeeb S . Effect of diurnal fasting on sleep during Ramadan: a systematic review and meta-analysis. Sleep Breath. 2019; 24(2):771-782. DOI: 10.1007/s11325-019-01986-1. View

Jablonska-Trypuc A, Pankiewicz W, Czerpak R . Traumatic Acid Reduces Oxidative Stress and Enhances Collagen Biosynthesis in Cultured Human Skin Fibroblasts. Lipids. 2016; 51(9):1021-35. PMC: 5009161. DOI: 10.1007/s11745-016-4174-5. View

10.

Kondoh H, Teruya T, Yanagida M . Metabolomics of human fasting: new insights about old questions. Open Biol. 2020; 10(9):200176. PMC: 7536077. DOI: 10.1098/rsob.200176. View

11.

Faris M, Jahrami H, BaHammam A, Kalaji Z, Madkour M, Hassanein M . A systematic review, meta-analysis, and meta-regression of the impact of diurnal intermittent fasting during Ramadan on glucometabolic markers in healthy subjects. Diabetes Res Clin Pract. 2020; 165:108226. DOI: 10.1016/j.diabres.2020.108226. View

12.

Faris M, Jahrami H, Abdelrahim D, Bragazzi N, BaHammam A . The effects of Ramadan intermittent fasting on liver function in healthy adults: A systematic review, meta-analysis, and meta-regression. Diabetes Res Clin Pract. 2021; 178:108951. DOI: 10.1016/j.diabres.2021.108951. View

13.

Jahrami H, Alsibai J, Clark C, Faris M . A systematic review, meta-analysis, and meta-regression of the impact of diurnal intermittent fasting during Ramadan on body weight in healthy subjects aged 16 years and above. Eur J Nutr. 2020; 59(6):2291-2316. DOI: 10.1007/s00394-020-02216-1. View

14.

Farooq A, Chamari K, Sayegh S, El Akoum M, Al-Mohannadi A . Ramadan daily intermittent fasting reduces objectively assessed habitual physical activity among adults. BMC Public Health. 2021; 21(1):1912. PMC: 8530011. DOI: 10.1186/s12889-021-11961-9. View

15.

Abdelrahim D, Faris M, Hassanein M, Shakir A, Yusuf A, Almeneessier A . Impact of Ramadan Diurnal Intermittent Fasting on Hypoglycemic Events in Patients With Type 2 Diabetes: A Systematic Review of Randomized Controlled Trials and Observational Studies. Front Endocrinol (Lausanne). 2021; 12:624423. PMC: 7984365. DOI: 10.3389/fendo.2021.624423. View

16.

Jahrami H, Faris M, Janahi A, Janahi M, Abdelrahim D, Madkour M . Does four-week consecutive, dawn-to-sunset intermittent fasting during Ramadan affect cardiometabolic risk factors in healthy adults? A systematic review, meta-analysis, and meta-regression. Nutr Metab Cardiovasc Dis. 2021; 31(8):2273-2301. DOI: 10.1016/j.numecd.2021.05.002. View

17.

Di Francesco A, Di Germanio C, Bernier M, de Cabo R . A time to fast. Science. 2018; 362(6416):770-775. PMC: 8504313. DOI: 10.1126/science.aau2095. View

18.

Shatila H, Baroudi M, Ahmad R, Chehab R, Forman M, Abbas N . Impact of Ramadan Fasting on Dietary Intakes Among Healthy Adults: A Year-Round Comparative Study. Front Nutr. 2021; 8:689788. PMC: 8375294. DOI: 10.3389/fnut.2021.689788. View

19.

Lee J, Verma N, Thakkar N, Yeung C, Sung H . Intermittent Fasting: Physiological Implications on Outcomes in Mice and Men. Physiology (Bethesda). 2020; 35(3):185-195. DOI: 10.1152/physiol.00030.2019. View

20.

Patterson R, Sears D . Metabolic Effects of Intermittent Fasting. Annu Rev Nutr. 2017; 37:371-393. DOI: 10.1146/annurev-nutr-071816-064634. View