Time-resolved RNA Signatures of CD4+ T Cells in Parkinson's Disease

Overview

Journal Cell Death Discov

Date 2023 Jan 21

PMID 36681665

Authors

Caroline Diener

Martin Hart

Tim Kehl

Anouck Becker-Dorison

Tanja Tanzer

David Schub

Lena Krammes

Martina Sester

Andreas Keller

Marcus Unger

Barbara Walch-Ruckheim

Hans-Peter Lenhof

Eckart Meese

Affiliations

Soon will be listed here.

Abstract

Parkinson's disease (PD) emerges as a complex, multifactorial disease. While there is increasing evidence that dysregulated T cells play a central role in PD pathogenesis, elucidation of the pathomechanical changes in related signaling is still in its beginnings. We employed time-resolved RNA expression upon the activation of peripheral CD4+ T cells to track and functionally relate changes on cellular signaling in representative cases of patients at different stages of PD. While only few miRNAs showed time-course related expression changes in PD, we identified groups of genes with significantly altered expression for each different time window. Towards a further understanding of the functional consequences, we highlighted pathways with decreased or increased activity in PD, including the most prominent altered IL-17 pathway. Flow cytometric analyses showed not only an increased prevalence of Th17 cells but also a specific subtype of IL-17 producing γδ-T cells, indicating a previously unknown role in PD pathogenesis.

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