Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2023 Jan 21

PMID 36679359

Authors

Tal Stern Bauer

Ravit Yakobi

Mattan Hurevich

Shlomo Yitzchaik

Zvi Hayouka

Affiliations

Soon will be listed here.

Abstract

The biosensing of bacterial pathogens is of a high priority. Electrochemical biosensors are an important future tool for rapid bacteria detection. A monolayer of bacterial-binding peptides can serve as a recognition layer in such detection devices. Here, we explore the potential of random peptide mixtures (RPMs) composed of phenylalanine and lysine in random sequences and of controlled length, to form a monolayer that can be utilized for sensing. RPMs were found to assemble in a thin and diluted layer that attracts various bacteria. Faradaic electrochemical impedance spectroscopy was used with modified gold electrodes to measure the charge-transfer resistance (R) caused due to the binding of bacteria to RPMs. was found to cause the most prominent increase in R compared to other model bacteria. We show that the combination of highly accessible antimicrobial RPMs and electrochemical analysis can be used to generate a new promising line of bacterial biosensors.

Citing Articles

Revolutionizing food safety with electrochemical biosensors for rapid and portable pathogen detection.

Avila Oliveira B, Gomes R, de Carvalho A, Lima E, Pinto U, da Cunha L Braz J Microbiol. 2024; 55(3):2511-2525.

PMID: 38922532 PMC: 11405362. DOI: 10.1007/s42770-024-01427-6.

References

Cheriker H, Stern Bauer T, Oren Y, Nir S, Hayouka Z . Immobilized random peptide mixtures exhibit broad antimicrobial activity with high selectivity. Chem Commun (Camb). 2020; 56(75):11022-11025. DOI: 10.1039/d0cc04759g. View

Joshi P, Mervinetsky E, Solomon O, Chen Y, Yitzchaik S, Friedler A . Electrochemical biosensors based on peptide-kinase interactions at the kinase docking site. Biosens Bioelectron. 2022; 207:114177. DOI: 10.1016/j.bios.2022.114177. View

Bassetti M, Rello J, Blasi F, Goossens H, Sotgiu G, Tavoschi L . Systematic review of the impact of appropriate versus inappropriate initial antibiotic therapy on outcomes of patients with severe bacterial infections. Int J Antimicrob Agents. 2020; 56(6):106184. DOI: 10.1016/j.ijantimicag.2020.106184. View

Topman-Rakover S, Malach E, Burdman S, Hayouka Z . Antibacterial lipo-random peptide mixtures exhibit high selectivity and synergistic interactions. Chem Commun (Camb). 2020; 56(80):12053-12056. DOI: 10.1039/d0cc04493h. View

Wang Y, Ye Z, Ying Y . New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria. Sensors (Basel). 2012; 12(3):3449-71. PMC: 3376556. DOI: 10.3390/s120303449. View

Rajapaksha P, Elbourne A, Gangadoo S, Brown R, Cozzolino D, Chapman J . A review of methods for the detection of pathogenic microorganisms. Analyst. 2018; 144(2):396-411. DOI: 10.1039/c8an01488d. View

Stern T, Zelinger E, Hayouka Z . Random peptide mixtures inhibit and eradicate methicillin-resistant Staphylococcus aureus biofilms. Chem Commun (Camb). 2016; 52(44):7102-5. DOI: 10.1039/c6cc01438k. View

Hayouka Z, Chakraborty S, Liu R, Boersma M, Weisblum B, Gellman S . Interplay among subunit identity, subunit proportion, chain length, and stereochemistry in the activity profile of sequence-random peptide mixtures. J Am Chem Soc. 2013; 135(32):11748-51. PMC: 3856984. DOI: 10.1021/ja406231b. View

Vericat C, Vela M, Benitez G, Carro P, Salvarezza R . Self-assembled monolayers of thiols and dithiols on gold: new challenges for a well-known system. Chem Soc Rev. 2010; 39(5):1805-34. DOI: 10.1039/b907301a. View

10.

Serra-Burriel M, Keys M, Campillo-Artero C, Agodi A, Barchitta M, Gikas A . Impact of multi-drug resistant bacteria on economic and clinical outcomes of healthcare-associated infections in adults: Systematic review and meta-analysis. PLoS One. 2020; 15(1):e0227139. PMC: 6953842. DOI: 10.1371/journal.pone.0227139. View

11.

Sonohara R, Muramatsu N, Ohshima H, Kondo T . Difference in surface properties between Escherichia coli and Staphylococcus aureus as revealed by electrophoretic mobility measurements. Biophys Chem. 1995; 55(3):273-7. DOI: 10.1016/0301-4622(95)00004-h. View

12.

Hayouka Z, Bella A, Stern T, Ray S, Jiang H, Grovenor C . Binary Encoding of Random Peptide Sequences for Selective and Differential Antimicrobial Mechanisms. Angew Chem Int Ed Engl. 2017; 56(28):8099-8103. DOI: 10.1002/anie.201702313. View

13.

Epand R, Savage P, Epand R . Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochim Biophys Acta. 2007; 1768(10):2500-9. DOI: 10.1016/j.bbamem.2007.05.023. View

14.

Bennett R, Oh M, Kuo S, Belo Y, Maron B, Malach E . Random Peptide Mixtures as Safe and Effective Antimicrobials against and MRSA in Mouse Models of Bacteremia and Pneumonia. ACS Infect Dis. 2021; 7(3):672-680. PMC: 8880176. DOI: 10.1021/acsinfecdis.0c00871. View

15.

Caraway H, Lau J, Maron B, Oh M, Belo Y, Brill A . Antimicrobial Random Peptide Mixtures Eradicate Biofilms and Inhibit Mouse Models of Infection. Antibiotics (Basel). 2022; 11(3). PMC: 8944503. DOI: 10.3390/antibiotics11030413. View

16.

Mitik-Dineva N, Wang J, Truong V, Stoddart P, Malherbe F, Crawford R . Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus attachment patterns on glass surfaces with nanoscale roughness. Curr Microbiol. 2008; 58(3):268-73. DOI: 10.1007/s00284-008-9320-8. View

17.

Guo Q, Li F . Self-assembled alkanethiol monolayers on gold surfaces: resolving the complex structure at the interface by STM. Phys Chem Chem Phys. 2014; 16(36):19074-90. DOI: 10.1039/c4cp00596a. View

18.

Weiss E, Kaufman G, Kriebel J, Li Z, Schalek R, Whitesides G . Si/SiO2-templated formation of ultraflat metal surfaces on glass, polymer, and solder supports: their use as substrates for self-assembled monolayers. Langmuir. 2007; 23(19):9686-94. DOI: 10.1021/la701919r. View

19.

Stern Bauer T, Hayouka Z . Random mixtures of antimicrobial peptides inhibit bacteria associated with pasteurized bovine milk. J Pept Sci. 2018; 24(7):e3088. DOI: 10.1002/psc.3088. View

20.

Topman S, Tamir-Ariel D, Bochnic-Tamir H, Stern Bauer T, Shafir S, Burdman S . Random peptide mixtures as new crop protection agents. Microb Biotechnol. 2018; 11(6):1027-1036. PMC: 6196386. DOI: 10.1111/1751-7915.13258. View