Novel Synthetic Co-culture of and Using CO and in Situ Generated H for the Production of Caproic Acid Via Lactic Acid

Overview

Journal Eng Life Sci

Specialty Biomedical Engineering

Date 2023 Jan 9

PMID 36619880

Authors

Jan Herzog

Alexander Mook

Lotta Guhl

Miriam Baumler

Matthias H Beck

Dirk Weuster-Botz

Frank R Bengelsdorf

An-Ping Zeng

Affiliations

Soon will be listed here.

Abstract

is known to produce mainly acetate from CO and H, but the production of higher value chemicals is desired for the bioeconomy. Using chain-elongating bacteria, synthetic co-cultures have the potential to produce longer-chained products such as caproic acid. In this study, we present first results for a successful autotrophic co-cultivation of mutants and a wild-type strain in a stirred-tank bioreactor for the production of caproic acid from CO and H via the intermediate lactic acid. For autotrophic lactate production, a recombinant strain with a deleted Lct-dehydrogenase complex, which is encoded by the genes, and an inserted D-lactate dehydrogenase (LdhD) originating from , was used. Hydrogen for the process was supplied using an All-in-One electrode for in situ water electrolysis. Lactate concentrations as high as 0.5 g L were achieved with the AiO-electrode, whereas 8.1 g L lactate were produced with direct H sparging in a stirred-tank bioreactor. Hydrogen limitation was identified in the AiO process. However, with cathode surface area enlargement or numbering-up of the electrode and on-demand hydrogen generation, this process has great potential for a true carbon-negative production of value chemicals from CO.

Citing Articles

Lactate-mediated mixotrophic co-cultivation of Clostridium drakei and recombinant Acetobacterium woodii for autotrophic production of volatile fatty acids.

Mook A, Herzog J, Walther P, Durre P, Bengelsdorf F Microb Cell Fact. 2024; 23(1):213.

PMID: 39061103 PMC: 11282840. DOI: 10.1186/s12934-024-02481-3.

Microbial electrosynthesis: opportunities for microbial pure cultures.

Harnisch F, Deutzmann J, Boto S, Rosenbaum M Trends Biotechnol. 2024; 42(8):1035-1047.

PMID: 38431514 PMC: 11310912. DOI: 10.1016/j.tibtech.2024.02.004.

Lactate based caproate production with and process control of via lactate dependent electrolysis.

Herzog J, Mook A, Utesch T, Bengelsdorf F, Zeng A Front Bioeng Biotechnol. 2023; 11:1212044.

PMID: 37425355 PMC: 10327822. DOI: 10.3389/fbioe.2023.1212044.

The potential of biofuels from first to fourth generation.

Cavelius P, Engelhart-Straub S, Mehlmer N, Lercher J, Awad D, Bruck T PLoS Biol. 2023; 21(3):e3002063.

PMID: 36996247 PMC: 10063169. DOI: 10.1371/journal.pbio.3002063.

Novel synthetic co-culture of and using CO and in situ generated H for the production of caproic acid via lactic acid.

Herzog J, Mook A, Guhl L, Baumler M, Beck M, Weuster-Botz D Eng Life Sci. 2023; 23(1):e2100169.

PMID: 36619880 PMC: 9815077. DOI: 10.1002/elsc.202100169.

References

Liu H, Grot S, Logan B . Electrochemically assisted microbial production of hydrogen from acetate. Environ Sci Technol. 2005; 39(11):4317-20. DOI: 10.1021/es050244p. View

Call D, Merrill M, Logan B . High surface area stainless steel brushes as cathodes in microbial electrolysis cells. Environ Sci Technol. 2009; 43(6):2179-83. DOI: 10.1021/es803074x. View

Novak K, Neuendorf C, Kofler I, Kieberger N, Klamt S, Pflugl S . Blending industrial blast furnace gas with H enables Acetobacterium woodii to efficiently co-utilize CO, CO and H. Bioresour Technol. 2020; 323:124573. DOI: 10.1016/j.biortech.2020.124573. View

Liu B, Popp D, Muller N, Strauber H, Harms H, Kleinsteuber S . Three Novel Isolates Produce -Caproate and -Butyrate from Lactate: Comparative Genomics of Chain-Elongating Bacteria. Microorganisms. 2020; 8(12). PMC: 7764203. DOI: 10.3390/microorganisms8121970. View

Zhu X, Zhou Y, Wang Y, Wu T, Li X, Li D . Production of high-concentration -caproic acid from lactate through fermentation using a newly isolated bacterium CPB6. Biotechnol Biofuels. 2017; 10:102. PMC: 5399333. DOI: 10.1186/s13068-017-0788-y. View

Takors R, Kopf M, Mampel J, Bluemke W, Blombach B, Eikmanns B . Using gas mixtures of CO, CO and H as microbial substrates: the do's and don'ts of successful technology transfer from laboratory to production scale. Microb Biotechnol. 2018; 11(4):606-625. PMC: 6011938. DOI: 10.1111/1751-7915.13270. View

Kantzow C, Weuster-Botz D . Effects of hydrogen partial pressure on autotrophic growth and product formation of Acetobacterium woodii. Bioprocess Biosyst Eng. 2016; 39(8):1325-30. DOI: 10.1007/s00449-016-1600-2. View

Kremp F, Poehlein A, Daniel R, Muller V . Methanol metabolism in the acetogenic bacterium Acetobacterium woodii. Environ Microbiol. 2018; 20(12):4369-4384. DOI: 10.1111/1462-2920.14356. View

Kucek L, Nguyen M, Angenent L . Conversion of L-lactate into n-caproate by a continuously fed reactor microbiome. Water Res. 2016; 93:163-171. DOI: 10.1016/j.watres.2016.02.018. View

10.

Schoelmerich M, Katsyv A, Sung W, Mijic V, Wiechmann A, Kottenhahn P . Regulation of lactate metabolism in the acetogenic bacterium Acetobacterium woodii. Environ Microbiol. 2018; 20(12):4587-4595. DOI: 10.1111/1462-2920.14412. View

11.

Enzmann F, Stockl M, Zeng A, Holtmann D . Same but different-Scale up and numbering up in electrobiotechnology and photobiotechnology. Eng Life Sci. 2020; 19(2):121-132. PMC: 6999560. DOI: 10.1002/elsc.201800160. View

12.

Utesch T, Zeng A . A novel All-in-One electrolysis electrode and bioreactor enable better study of electrochemical effects and electricity-aided bioprocesses. Eng Life Sci. 2020; 18(8):600-610. PMC: 6999482. DOI: 10.1002/elsc.201700198. View

13.

Herzog J, Mook A, Guhl L, Baumler M, Beck M, Weuster-Botz D . Novel synthetic co-culture of and using CO and in situ generated H for the production of caproic acid via lactic acid. Eng Life Sci. 2023; 23(1):e2100169. PMC: 9815077. DOI: 10.1002/elsc.202100169. View

14.

Arbter P, Sinha A, Troesch J, Utesch T, Zeng A . Redox governed electro-fermentation improves lipid production by the oleaginous yeast Rhodosporidium toruloides. Bioresour Technol. 2019; 294:122122. DOI: 10.1016/j.biortech.2019.122122. View

15.

Kusel K, Dorsch T, Acker G, Stackebrandt E, Drake H . Clostridium scatologenes strain SL1 isolated as an acetogenic bacterium from acidic sediments. Int J Syst Evol Microbiol. 2000; 50 Pt 2:537-546. DOI: 10.1099/00207713-50-2-537. View

16.

Detman A, Mielecki D, Chojnacka A, Salamon A, Blaszczyk M, Sikora A . Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors. Microb Cell Fact. 2019; 18(1):36. PMC: 6373154. DOI: 10.1186/s12934-019-1085-1. View

17.

Baumler M, Schneider M, Ehrenreich A, Liebl W, Weuster-Botz D . Synthetic co-culture of autotrophic Clostridium carboxidivorans and chain elongating Clostridium kluyveri monitored by flow cytometry. Microb Biotechnol. 2021; 15(5):1471-1485. PMC: 9049614. DOI: 10.1111/1751-7915.13941. View

18.

Neuendorf C, Vignolle G, Derntl C, Tomin T, Novak K, Mach R . A quantitative metabolic analysis reveals Acetobacterium woodii as a flexible and robust host for formate-based bioproduction. Metab Eng. 2021; 68:68-85. DOI: 10.1016/j.ymben.2021.09.004. View

19.

Schneider M, Baumler M, Lee N, Weuster-Botz D, Ehrenreich A, Liebl W . Monitoring co-cultures of Clostridium carboxidivorans and Clostridium kluyveri by fluorescence in situ hybridization with specific 23S rRNA oligonucleotide probes. Syst Appl Microbiol. 2021; 44(6):126271. DOI: 10.1016/j.syapm.2021.126271. View

20.

Bordbar A, Monk J, King Z, Palsson B . Constraint-based models predict metabolic and associated cellular functions. Nat Rev Genet. 2014; 15(2):107-20. DOI: 10.1038/nrg3643. View