Recent Advances in Producing Food Additive L-malate: Chassis, Substrate, Pathway, Fermentation Regulation and Application

Overview

Journal Microb Biotechnol

Specialties Biotechnology
Microbiology

Date 2023 Jan 5

PMID 36604311

Authors

Qiang Ding

Chao Ye

Affiliations

Soon will be listed here.

Abstract

In addition to being an important intermediate in the TCA cycle, L-malate is also widely used in the chemical and beverage industries. Due to the resulting high demand, numerous studies investigated chemical methods to synthesize L-malate from petrochemical resources, but such approaches are hampered by complex downstream processing and environmental pollution. Accordingly, there is an urgent need to develop microbial methods for environmentally-friendly and economical L-malate biosynthesis. The rapid progress and understanding of DNA manipulation, cell physiology, and cell metabolism can improve industrial L-malate biosynthesis by applying intelligent biochemical strategies and advanced synthetic biology tools. In this paper, we mainly focused on biotechnological approaches for enhancing L-malate synthesis, encompassing the microbial chassis, substrate utilization, synthesis pathway, fermentation regulation, and industrial application. This review emphasizes the application of novel metabolic engineering strategies and synthetic biology tools combined with a deep understanding of microbial physiology to improve industrial L-malate biosynthesis in the future.

Citing Articles

Recent advances in producing food additive L-malate: Chassis, substrate, pathway, fermentation regulation and application.

Ding Q, Ye C Microb Biotechnol. 2023; 16(4):709-725.

PMID: 36604311 PMC: 10034640. DOI: 10.1111/1751-7915.14206.

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